Cho L, Tsunoda M, Sharma R P
Department of Physiology and Pharmacology, College of Veterinary Medicine, The University of Georgia, Athens 30602-7389, USA.
Nat Toxins. 1999;7(5):187-95. doi: 10.1002/1522-7189(200009/10)7:5<187::AID-NT58>3.0.CO;2-1.
Alterations in regional brain concentration of dopamine (DA), norepinephrine (NE), serotonin (5-HT) and their metabolites were investigated in male BALB/c mice injected intraperitoneally with bacterial lipopolysaccharide (LPS, 2 mg kg(-1)) or recombinant murine tumor necrosis factor alpha (TNFalpha, 0.1 mg kg(-1)) at 2, 6, 12 and 24 h after the injection. At 2 h post-injection the LPS administration resulted in hypothermia, which was not apparent at later time points. No consistent effects were observed by either LPS or TNFalpha on peripheral leukocyte counts or plasma transaminase levels. Both LPS and TNFalpha slightly elevated NE metabolism in the striatum at 2-12 h. Concentrations of DA and its metabolites were significantly elevated only in the hypothalamus following TNFalpha at 24 h. Tumor necrosis factor alpha exerted pronounced effects on 5-HT metabolism in most brain regions at 2 h. Results suggest that the effect of LPS is more complex compared with TNFalpha because of the endogenous production of other cytokines including the TNFalpha.
在雄性BALB/c小鼠腹腔注射细菌脂多糖（LPS，2 mg kg⁻¹）或重组小鼠肿瘤坏死因子α（TNFα，0.1 mg kg⁻¹）后2、6、12和24小时，研究了大脑不同区域多巴胺（DA）、去甲肾上腺素（NE）、血清素（5-HT）及其代谢产物浓度的变化。注射后2小时，LPS给药导致体温过低，在随后的时间点未观察到明显变化。LPS或TNFα对外周白细胞计数或血浆转氨酶水平均未观察到一致的影响。LPS和TNFα在2 - 12小时均使纹状体中的NE代谢略有升高。仅在TNFα注射24小时后，下丘脑的DA及其代谢产物浓度显著升高。肿瘤坏死因子α在2小时时对大多数脑区的5-HT代谢产生显著影响。结果表明，与TNFα相比，LPS的作用更为复杂，这是因为包括TNFα在内的其他细胞因子的内源性产生。
