Kuiper R P, Martens G J
Department of Animal Physiology, University of Nijmegen, The Netherlands.
Biochem Cell Biol. 2000;78(3):289-98.
En route through the secretory pathway of neuroendocrine cells, prohormones pass a series of membrane-bounded compartments. During this transport, the prohormones are sorted to secretory granules and proteolytically cleaved to bioactive peptides. Recently, progress has been made in a number of aspects concerning secretory protein transport and sorting, particularly with respect to transport events in the early regions of the secretory pathway. In this review we will deal with some of these aspects, including: i) selective exit from the endoplasmic reticulum via COPII-coated vesicles and the potential role of p24 putative cargo receptors in this process, ii) cisternal maturation as an alternative model for protein transport through the Golgi complex, and iii) the mechanisms that may be involved in the sorting of regulated secretory proteins to secretory granules. Although much remains to be learned, interesting new insights into the functioning of the secretory pathway have been obtained.
在通过神经内分泌细胞的分泌途径的过程中，激素原会经过一系列膜结合的区室。在这种转运过程中，激素原被分选到分泌颗粒中，并被蛋白水解切割成生物活性肽。最近，在分泌蛋白转运和分选的多个方面取得了进展，特别是在分泌途径早期区域的转运事件方面。在这篇综述中，我们将探讨其中的一些方面，包括：i）通过COPII包被小泡从内质网的选择性输出以及p24假定货物受体在此过程中的潜在作用，ii）作为蛋白质通过高尔基体复合体转运的替代模型的潴泡成熟，以及iii）可能参与将受调节的分泌蛋白分选到分泌颗粒中的机制。尽管仍有许多有待了解的地方，但已经获得了关于分泌途径功能的有趣新见解。
