Willems R J, Top J, van Den Braak N, van Belkum A, Endtz H, Mevius D, Stobberingh E, van Den Bogaard A, van Embden J D
Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, 3720 BA Bilthoven, The Netherlands.
J Infect Dis. 2000 Sep;182(3):816-23. doi: 10.1086/315752. Epub 2000 Aug 10.
Amplified-fragment length polymorphism (AFLP) analysis was used to investigate the genetic relationships among 255 vancomycin-resistant Enterococcus faecium (VREF) strains isolated from hospitalized patients, nonhospitalized persons, and various animal sources. Four major AFLP genogroups (A-D) were discriminated. The strains of each taxon shared >/=65% of the restriction fragments. Most isolates recovered from nonhospitalized persons (75%) were grouped together with all pig isolates in genogroup A. Most isolates from hospitalized patients (84%), a subset of veal calf isolates (25%), and all isolates from cats and dogs clustered in genogroup C. Most isolates from chickens (97%) and turkeys (86%) were grouped in genogroup B, whereas most veal calf isolates (70%) clustered in genogroup D. Therefore, VREF strains are predominantly host-specific, and strains isolated from hospitalized patients are genetically different from the prevailing VREF strains present in the fecal flora of nonhospitalized persons.
扩增片段长度多态性（AFLP）分析用于研究从住院患者、非住院人员及各种动物来源分离出的255株耐万古霉素屎肠球菌（VREF）之间的遗传关系。鉴别出四个主要的AFLP基因组群（A - D）。每个分类单元的菌株共享≥65%的限制性片段。从非住院人员中分离出的大多数菌株（75%）与A基因组群中的所有猪源菌株归为一组。从住院患者中分离出的大多数菌株（84%）、小牛肉牛犊分离株的一个子集（25%）以及从猫和狗中分离出的所有菌株聚集在C基因组群。从鸡（97%）和火鸡（86%）中分离出的大多数菌株归为B基因组群，而大多数小牛肉牛犊分离株（70%）聚集在D基因组群。因此，VREF菌株主要具有宿主特异性，并且从住院患者中分离出的菌株在遗传上与非住院人员粪便菌群中占主导地位的VREF菌株不同。
