Isales C M, Sumpio B, Bollag R J, Zhong Q, Ding K H, Du W, Rodriguez-Commes J, Lopez R, Rosales O R, Gasalla-Herraiz J, McCarthy R, Barrett P Q
Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta 30912, USA.
Am J Physiol Endocrinol Metab. 2000 Sep;279(3):E654-62. doi: 10.1152/ajpendo.2000.279.3.E654.
Acute parathyroid hormone exposure induces vascular smooth muscle relaxation. In contrast, continuous infusion of parathyroid hormone leads to vasoconstriction and an elevation of blood pressure. Despite the known effects of parathyroid hormone on vascular smooth muscle, possible direct effects on the vascular endothelium have not previously been investigated. Using a human umbilical vein endothelial cell line, we found that parathyroid hormone increased both intracellular calcium and cellular cAMP content in these endothelial cells. Furthermore, exposure of these cells to increasing concentrations of parathyroid hormone stimulated both [(3)H]thymidine incorporation and endothelin-1 secretion. Parathyroid hormone/parathyroid hormone-related peptide receptor mRNA could be detected at low levels in these cells. In summary, these data demonstrate that endothelium-derived cells contain functional parathyroid hormone receptors. The potential physiological role of these receptors remains to be determined.
急性甲状旁腺激素暴露可诱导血管平滑肌舒张。相比之下，持续输注甲状旁腺激素会导致血管收缩和血压升高。尽管已知甲状旁腺激素对血管平滑肌有作用，但此前尚未研究其对血管内皮可能的直接作用。使用人脐静脉内皮细胞系，我们发现甲状旁腺激素可增加这些内皮细胞内的钙含量和细胞环磷酸腺苷（cAMP）水平。此外，将这些细胞暴露于浓度递增的甲状旁腺激素中会刺激[³H]胸腺嘧啶核苷掺入及内皮素-1分泌。在这些细胞中可检测到低水平的甲状旁腺激素/甲状旁腺激素相关肽受体信使核糖核酸（mRNA）。总之，这些数据表明内皮衍生细胞含有功能性甲状旁腺激素受体。这些受体的潜在生理作用仍有待确定。
