Desclin J C, Escubi J
Brain Res. 1975 Jul 25;93(1):25-39. doi: 10.1016/0006-8993(75)90284-x.
A new silver impregnation technique for frozen and paraffin sections of formalin-fixed nervous tissue has been devised by modifying the Fink-Heimer14 and De Olmos-Ingram6 methods. The modified versions have been combined into a single staining procedure. Cobalt or lead nitrate are substituted for uranyl nitrate. Silver nitrate in the impregnating solution is replaced by silver lactate or protargol. This procedure results in a preferential impregnation of degenerating perikarya, dendrites, axons and synaptic endings. It affords a more complete impregnation of 'preterminal' axonal arborizations than previous methods. The method is effective for the demonstration of degenerating terminals in the rat cerebellar cortex. Emphasis has been laid on the usefulness of the method for the study of drug-induced CNS lesions.
通过改进芬克-海默14法和德奥尔莫斯-英格拉姆6法，设计出了一种用于福尔马林固定神经组织冰冻切片和石蜡切片的新银浸染技术。改进后的方法已合并为单一染色程序。用硝酸钴或硝酸铅替代硝酸铀酰。浸染溶液中的硝酸银被乳酸银或蛋白银取代。该程序可优先浸染变性的核周体、树突、轴突和突触末梢。与以往方法相比，它能更完整地浸染“终末前”轴突分支。该方法对显示大鼠小脑皮质中的变性终末有效。重点强调了该方法在研究药物诱导的中枢神经系统损伤方面的实用性。
