粗糙脉孢菌het-c异核体不相容基因在嗜鸟粪盘菌中的同源基因hch的特性分析

Characterization of hch, the Podospora anserina homolog of the het-c heterokaryon incompatibility gene of Neurospora crassa.

作者信息

Saupe S J, Clavé C, Sabourin M, Bégueret J

机构信息

Laboratoire de Génétique Moléculaire des Champignons Filamenteux, IBGC UMR CNRS, Bordeaux, France.

出版信息

Curr Genet. 2000 Jul;38(1):39-47. doi: 10.1007/s002940000130.

DOI:10.1007/s002940000130

PMID:10953880

Abstract

The het-c locus controls heterokaryon formation in Neurospora crassa. It is subject to balancing selection operating to maintain polymorphism at that locus in natural populations. We have isolated hch, the het-c homolog from the related species Podospora anserina (hch for het-c homolog), in order to determine if this gene also functions as a het gene in that species. The het-c and hch sequences are highly similar but differ in the region defining allele specificity in N. crassa het-c. Analysis of hch variability in 11 natural P. anserina isolates with different het genotypes revealed no polymorphism. This suggested that hch does not function as a het gene. However, heterologous expression of the N. crassa het-cPA allele in P. anserina triggers a growth defect reminiscent of the het-c incompatibility reaction.

摘要

het-c基因座控制粗糙脉孢菌中的异核体形成。它受到平衡选择的作用，以维持自然种群中该基因座的多态性。我们从相关物种嗜热栖热放线菌中分离出了hch，即het-c的同源基因（hch代表het-c同源基因），以确定该基因在该物种中是否也作为het基因发挥作用。het-c和hch序列高度相似，但在定义粗糙脉孢菌het-c等位基因特异性的区域有所不同。对11个具有不同het基因型的嗜热栖热放线菌自然分离株中的hch变异性分析显示没有多态性。这表明hch不作为het基因发挥作用。然而，粗糙脉孢菌het-cPA等位基因在嗜热栖热放线菌中的异源表达引发了一种生长缺陷，让人联想到het-c不相容反应。