Rebeiz M, Lewin H A
Department of Animal Sciences, The W.M. Keck Center for Comparative and Functional Genomics, University of Illinois at Urbana-Champaign, 61801, USA.
Anim Biotechnol. 2000 Nov;11(2):75-241. doi: 10.1080/10495390009525951.
Comparative Mapping by Annotation and Sequence Similarity (COMPASS) has been demonstrated to be an effective approach for predicting the chromosome location of expressed sequence tags (ESTs) and other sequence-based markers on the basis of comparative mapping information. Herein, we describe the development and use of a computer program to execute the COMPASS strategy en masse. The program was used to identify orthologs and predict map locations of 47,787 cattle ESTs. Among these 47,787 ESTs, 30,097 had significant matches with sequences in the human UniGene database and 21,311 were annotated with human GB4 radiation hybrid mapping data. These sequences are contained within 9,956 and 6,295 individual human UniGene clusters, respectively. The putative human orthologs and predicted cattle chromosome locations of the 21,311 cattle ESTs with GB4 mapping data are provided in this report as a resource for the research community.
通过注释和序列相似性进行比较定位（COMPASS）已被证明是一种基于比较定位信息预测表达序列标签（EST）和其他基于序列的标记物染色体位置的有效方法。在此，我们描述了一个计算机程序的开发和使用，以便大规模执行COMPASS策略。该程序用于识别直系同源物并预测47,787个牛EST的图谱位置。在这47,787个EST中，30,097个与人类UniGene数据库中的序列有显著匹配，21,311个用人类GB4辐射杂种图谱数据进行了注释。这些序列分别包含在9,956个和6,295个单独的人类UniGene簇中。本报告提供了具有GB4图谱数据的21,311个牛EST的推定人类直系同源物和预测的牛染色体位置，作为研究界的一种资源。
