化脓性肉芽肿中细胞凋亡及Bax/Bcl-2蛋白表达：与肉芽组织和毛细血管瘤的对比研究
Apoptosis and expression of Bax/Bcl-2 proteins in pyogenic granuloma: a comparative study with granulation tissue and capillary hemangioma.
作者信息
Nakamura T
机构信息
Department of Pathology, Suwa Red Cross Hospital, Japan.
出版信息
J Cutan Pathol. 2000 Sep;27(8):400-5. doi: 10.1034/j.1600-0560.2000.027008400.x.
BACKGROUND
Pyogenic granuloma (PG) has been regarded as a florid expression of granulation tissue (GT) proliferation and shows distinct biological behavior compared to conventional GT. PG has not been examined from a standpoint of apoptosis.
METHODS
PG (15 cases), GT (14 cases) and capillary hemangioma (CH, 8 cases) were compared using in situ 3'-tailing reaction (ISTR), a histochemical method for identifying apoptotic cells, and immunohistochemistry for Ki-67, Bcl-2 and Bax proteins, in order to clarify and involvement of apoptosis in the difference of biological characteristics between PG and GT.
RESULTS
PG showed significantly lower ISTR-labeling indices than GT and CH and more frequent Bcl-2/Bax expression than GT, whereas Ki-67-labeling indices were variable from case to case and did not show any significant differences among any groups.
CONCLUSIONS
It is consequently suggested that the low apoptotic rate in PG is closely related to its characteristic rapid growth and is regulated by Bcl-2 family proteins.
背景
化脓性肉芽肿（PG）被认为是肉芽组织（GT）增殖的一种旺盛表现，与传统的GT相比具有独特的生物学行为。尚未从细胞凋亡的角度对PG进行研究。
方法
使用原位3'-末端加尾反应（ISTR，一种用于识别凋亡细胞的组织化学方法）以及对Ki-67、Bcl-2和Bax蛋白进行免疫组织化学，对15例PG、14例GT和8例毛细血管瘤（CH）进行比较，以阐明细胞凋亡与PG和GT生物学特性差异之间的关系。
结果
PG的ISTR标记指数显著低于GT和CH，且Bcl-2/Bax表达比GT更频繁，而Ki-67标记指数因病例而异，在任何组之间均未显示出任何显著差异。
结论
因此，提示PG中低凋亡率与其特征性的快速生长密切相关，并受Bcl-2家族蛋白调节。
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