Weisbart R H, Bluestone R, Goldberg L S
J Lab Clin Med. 1975 Jan;85(1):87-92.
Cellular immunity to hog intrinsic factor was detected by a modified agarose-leukocyte migration test in 18 patients with pernicious amemia. Lymphocytes from 17 out of 18 patients with pernicious anemia gave positive responses to a concentrate of hog intrinsic factor; the intrinsic factor present in 1 mg. of this concentrate bound 128 ng. of vatamin B12. Six patients with atrophic gastritis, 7 with regional enteritis, and 9 out of 10 healthy adults did not respond to this preparation. No correlation existed between the presence of serum autoantibodies to intrinsic factor and in vitro lymphocyte responsiveness to intrinsic factor. The results demonstrate that cellular immunity to intrinsic factor concentrates is present in the majority of patients with pernicious anemia.
采用改良的琼脂糖-白细胞迁移试验，检测了18例恶性贫血患者对猪内因子的细胞免疫。18例恶性贫血患者中有17例的淋巴细胞对猪内因子浓缩物呈阳性反应；1毫克该浓缩物中的内因子可结合128纳克维生素B12。6例萎缩性胃炎患者、7例局限性肠炎患者以及10名健康成年人中的9人对该制剂无反应。血清中内因子自身抗体的存在与体外淋巴细胞对内因子的反应性之间无相关性。结果表明，大多数恶性贫血患者存在对内因子浓缩物的细胞免疫。
