Séguier S, Godeau G, Brousse N
Service d'Anatomie et de Cytologie Pathologiques, Hôpital Necker-Enfants Malades, Paris, France.
J Periodontol. 2000 Jul;71(7):1079-85. doi: 10.1902/jop.2000.71.7.1079.
Periodontal disease is histologically characterized by the degradation of extracellular matrix components associated with a gingival infiltration of inflammatory cell populations. The purpose of this in situ study was to quantify inflammatory cell subsets and the area fraction (AA%) occupied by collagen fibers in healthy and diseased upper gingival connective tissue in order to investigate the association, if any, between collagen loss and inflammatory cell infiltrate.
Paraffin gingival tissue sections from 10 healthy controls (C), 9 patients with gingivitis (G), and 10 patients with severe chronic periodontitis (P) were immunohistochemically stained by antibodies against CD45, CD3, CD8, CD20, CD68, TIA-1, and GrB molecules, and the collagen fibers were stained using sirius red F3Ba. The quantitative evaluations of inflammatory cell numbers and the AA% occupied by collagen fibers were performed by morphometric and automated image analysis.
In group P, CD45+, CD20+, CD68+, TIA-1+, and GrB+ cell numbers were significantly increased (P<0.05) when compared to both C and G groups. The present study revealed significant differences (P <0.01) between means of AA% observed in group C (63%), group G (46%), and group P (26%), and AA% of group G and group P was inversely correlated with the numbers of TIA-1+ cells (P<0.01) and GrB+ cells (P<0.01 and P<0.05, respectively).
This study showed great differences in the number of the distinct inflammatory cell subsets according to the severity of the periodontal disease and suggested that activated cytotoxic cells could play a pivotal role in the loss of collagen fibers observed during these pathological states. During periodontitis, collagen loss was significantly correlated with all inflammatory cell subset numbers. Finally, the quantitative evaluation of the area fraction occupied by gingival collagen fibers may reflect the clinical severity of the periodontal disease.
牙周病在组织学上的特征是细胞外基质成分降解，伴有炎症细胞群体浸润牙龈。本原位研究的目的是量化健康和患病上颌牙龈结缔组织中炎症细胞亚群以及胶原纤维所占的面积分数（AA%），以研究胶原丢失与炎症细胞浸润之间是否存在关联。
对10名健康对照者（C组）、9名牙龈炎患者（G组）和10名重度慢性牙周炎患者（P组）的石蜡包埋牙龈组织切片进行免疫组织化学染色，使用抗CD45、CD3、CD8、CD20、CD68、TIA-1和GrB分子的抗体，并用天狼星红F3Ba对胶原纤维进行染色。通过形态计量学和自动图像分析对炎症细胞数量和胶原纤维所占的AA%进行定量评估。
与C组和G组相比，P组中CD45+、CD20+、CD68+、TIA-1+和GrB+细胞数量显著增加（P<0.05）。本研究显示C组（63%）、G组（46%）和P组（26%）观察到的AA%平均值之间存在显著差异（P<0.01），G组和P组的AA%与TIA-1+细胞数量（P<0.01）和GrB+细胞数量（分别为P<0.01和P<0.05）呈负相关。
本研究表明，根据牙周病的严重程度，不同炎症细胞亚群的数量存在很大差异，并提示活化的细胞毒性细胞可能在这些病理状态下观察到的胶原纤维丢失中起关键作用。在牙周炎期间，胶原丢失与所有炎症细胞亚群数量显著相关。最后，牙龈胶原纤维所占面积分数的定量评估可能反映牙周病的临床严重程度。
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