Antona María Eugenia, Ramos Cecilia, Orzuza Ricardo, González Germán Esteban, González Paula Mariela, Cabrera Joaquín, Ferreira Monteiro Andrea Gloria, Zago Valeria, Friedman Silvia María, Steimetz Tammy, Macri Elisa Vanesa
Facultad de Odontología. Cátedra de Bioquímica General y Bucal., Universidad de Buenos Aires, Marcelo T. de Alvear 2142 12 B, (C1122 AAH), Buenos Aires, Argentina.
Facultad de Medicina, Instituto de Investigaciones Biomédicas (UCA-CONICET), Laboratorio de Patología Cardiovascular Experimental e Hipertensión Arterial, Pontificia Universidad Católica Argentina, Buenos Aires, Argentina.
Odontology. 2024 Dec 20. doi: 10.1007/s10266-024-01042-9.
This study investigated the preventive effect of curcumin (CUR) on tooth-supporting structures in hypercholesterolemic (HC) rats with periodontitis (P). Wistar rats (8 weeks old) (n = 30) were assigned to six groups based on dietary intake, CUR-piperine combination treatment and P induction. P was induced in four groups using a ligature model. Serum lipid profiles, oxidative stress parameters, radiographic, histological and histomorphometric analyses were performed. HC rats showed elevated serum cholesterol levels (p < 0.001). Moreover, topical administration of CUR did not regulate hypercholesterolemia in this model. The HC diet increased oxidative stress in gingival tissue, exacerbated by P, whereas CUR attenuated reactive species generation (p < 0.001) and reduced catalase (CAT) activity, possibly due to its antioxidant properties. Histological analysis revealed extensive erosive surfaces and osteoclast presence in the P groups, with the HC + P group showing the highest rate of bone resorption. The CUR-treated groups showed less bone resorption and more bone formation, indicating a protective effect. Histomorphometric studies showed a significant increase in bone volume in the CUR groups compared to the P groups (p < 0.001). CUR prevented bone resorption induced by P and HC diet, with larger osteoblastic surfaces and fewer osteoclasts, suggesting inhibition of bone resorption. CUR also prevented collagen fiber destruction caused by the HC diet. Overall, the study suggests a potential therapeutic role for CUR in mitigating periodontal tissue damage associated with hypercholesterolemia and P, due to its antioxidant and anti-inflammatory properties. Further research would be needed to validate its clinical efficacy as an adjunctive treatment for P.
本研究调查了姜黄素（CUR）对患有牙周炎（P）的高胆固醇血症（HC）大鼠牙齿支持结构的预防作用。将8周龄的Wistar大鼠（n = 30）根据饮食摄入、CUR-胡椒碱联合治疗和P诱导分为六组。使用结扎模型在四组中诱导P。进行了血清脂质谱、氧化应激参数、影像学、组织学和组织形态计量学分析。HC大鼠的血清胆固醇水平升高（p < 0.001）。此外，在该模型中，局部施用CUR并未调节高胆固醇血症。HC饮食增加了牙龈组织中的氧化应激，P使其加剧，而CUR减弱了活性物质的产生（p < 0.001）并降低了过氧化氢酶（CAT）活性，这可能归因于其抗氧化特性。组织学分析显示P组有广泛的糜烂表面和破骨细胞存在，HC + P组显示出最高的骨吸收速率。CUR治疗组的骨吸收较少且骨形成较多，表明具有保护作用。组织形态计量学研究显示，与P组相比，CUR组的骨体积显著增加（p < 0.001）。CUR预防了由P和HC饮食诱导的骨吸收，成骨表面更大且破骨细胞更少，表明抑制了骨吸收。CUR还预防了HC饮食引起的胶原纤维破坏。总体而言，该研究表明，由于其抗氧化和抗炎特性，CUR在减轻与高胆固醇血症和P相关的牙周组织损伤方面具有潜在的治疗作用。需要进一步研究以验证其作为P辅助治疗的临床疗效。
