Sattelle D B, Harrison J B, Chen H H, Bai D, Takeda M
MRC Functional Genetics Unit, Department of Human Anatomy & Genetics, University of Oxford, UK.
Neurosci Lett. 2000 Aug 11;289(3):197-200. doi: 10.1016/s0304-3940(00)01292-1.
A polyclonal antibody raised against a 17 amino acid polypeptide (the predicted C-terminal sequence of the cloned Drosophila melanogaster gamma-aminobutyric acid (GABA) receptor subunit, RDL) was used to investigate the distribution of GABA receptor subunit(s) of this type in the nervous system of the cockroach Periplaneta americana. Intense staining was detected in the calyces of the mushroom bodies, glomeruli of the antennal lobes, lower central body, the corpora cardiaca and several cell layers of the medulla and the lobula regions of the optic lobe. The most intense immunocytochemical staining was in the suboesophageal ganglion. Control sections pre-incubated with the primary antibody and conjugated peptide were not stained. Thus, it appears that a GABA receptor subunit of the RDL type is located in cockroach brain regions involved in the processing of visual, olfactory and mechanosensory inputs to the nervous system. Since the corpora cardiaca reacted to this antiserum, this type of GABA receptor may also be involved in the regulation of neurosecretory activity.
一种针对17个氨基酸多肽（克隆的黑腹果蝇γ-氨基丁酸（GABA）受体亚基RDL的预测C端序列）产生的多克隆抗体，用于研究这种类型的GABA受体亚基在美洲大蠊神经系统中的分布。在蘑菇体的萼、触角叶的小球、下中枢体、心侧体以及视叶髓质和小叶区域的几个细胞层中检测到强烈染色。免疫细胞化学染色最强烈的是在咽下神经节。用一抗和偶联肽预孵育的对照切片未染色。因此，似乎RDL型GABA受体亚基位于蟑螂大脑中参与处理神经系统视觉、嗅觉和机械感觉输入的区域。由于心侧体对这种抗血清有反应，这种类型的GABA受体也可能参与神经分泌活动的调节。
