Jirón M I, Ardiles A, Parra M A, Orellana J
Departamento de Medicina, Facultad de Medicina, Universidad de Chile.
Rev Med Chil. 2000 Mar;128(3):273-8.
Anti liver kidney microsome antibodies (LKM-1) have been recently incorporated to the study and classification of chronic autoimmune hepatitis (HC-A1). The presence of anti LKM-1 antibodies and essential cryoglobulinemia is frequent in virus C associated chronic hepatitis (HC-VC).
To study the frequency of anti LKM-1 antibodies and cryoglobulin levels in patients with HC-AI, HC-VC and cryptogenic cirrhosis.
Forty-two patients were studied. Nineteen adult women with classic HC-AI with positive antinuclear or anti smooth muscle antibodies. Five patients of less than 20 years old with HC-AI and negative antinuclear and anti smooth muscle antibodies. Ten adult women with cryptogenic cirrhosis, 4 women and 4 men with HC-VC. Serum samples were obtained at 37 degrees C. Antinuclear, anti smooth muscle and anti LKM-1 antibodies were measured by indirect immunofluorescence using Hep-2 cells and rat tissue slices as substrates. Cryoglobulins were determined by the traditional method and cryocrit.
All studied patients were anti LKM-1 negative. All had significant circulating cryoglobulin levels.
In this sample of patients with HC-AI or HC-VC, anti LKM-1 antibodies were not detected but all had cryoglobulinemia.
抗肝肾微粒体抗体（LKM-1）最近已被纳入慢性自身免疫性肝炎（HC-A1）的研究和分类中。抗LKM-1抗体的存在和原发性冷球蛋白血症在丙型肝炎病毒相关的慢性肝炎（HC-VC）中很常见。
研究HC-AI、HC-VC和隐源性肝硬化患者中抗LKM-1抗体的频率和冷球蛋白水平。
研究了42例患者。19名成年女性患有经典HC-AI，抗核抗体或抗平滑肌抗体呈阳性。5名年龄小于20岁的HC-AI患者，抗核抗体和抗平滑肌抗体呈阴性。10名成年女性患有隐源性肝硬化，4名女性和4名男性患有HC-VC。在37摄氏度下采集血清样本。使用Hep-2细胞和大鼠组织切片作为底物，通过间接免疫荧光法测量抗核抗体、抗平滑肌抗体和抗LKM-1抗体。通过传统方法和冷球蛋白比容测定冷球蛋白。
所有研究患者抗LKM-1均为阴性。所有患者循环冷球蛋白水平均显著升高。
在该HC-AI或HC-VC患者样本中，未检测到抗LKM-1抗体，但所有患者均有冷球蛋白血症。
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