Nakamura Y, Miura K, Fujino Y, Iwao H, Ogita S, Yamanaka S
Department of Obstetrics and Gynecology, Osaka City University Medical School, Osaka, 545-8585, Japan.
Genomics. 2000 Sep 1;68(2):179-86. doi: 10.1006/geno.2000.6287.
Oscillin was identified from hamster sperm as a factor responsible for oocyte calcium oscillations. However, its high level of homology with the bacterial glucosamine-6-phosphate isomerase suggests that it may play more fundamental roles. In the current study, we identified Oscillin orthologs from Caenorhabditis elegans, Drosophila melanogaster, mouse, and human. Their amino acid identities with hamster oscillin were 67.0, 72.3, 97.6, and 95.5%, respectively. No Oscillin orthologs were found in Saccharomyces cerevisiae. The human Oscillin gene (HGMW-approved symbol GNPI) spans 12.4 kb and consists of eight exons. The position of the fourth intron was conserved in other species. The human Oscillin promoter has features characteristic of housekeeping genes, including a GC-rich content, multiple SP1 binding sites, and the absence of a TATA motif. Human and mouse Oscillin genes were ubiquitously expressed in all tissues examined. These data showed that Oscillin is a housekeeping gene conserved throughout evolution and do not support the notion that Oscillin is the sperm-specific factor responsible for calcium oscillations.
振荡素是从仓鼠精子中鉴定出的一种导致卵母细胞钙振荡的因子。然而，它与细菌葡糖胺-6-磷酸异构酶的高度同源性表明它可能发挥更基本的作用。在本研究中，我们从秀丽隐杆线虫、黑腹果蝇、小鼠和人类中鉴定出了振荡素直系同源物。它们与仓鼠振荡素的氨基酸同一性分别为67.0%、72.3%、97.6%和95.5%。在酿酒酵母中未发现振荡素直系同源物。人类振荡素基因（HGMW批准的符号为GNPI）跨度为12.4 kb，由八个外显子组成。第四个内含子的位置在其他物种中是保守的。人类振荡素启动子具有管家基因的特征，包括富含GC的含量、多个SP1结合位点以及没有TATA基序。人类和小鼠的振荡素基因在所检测的所有组织中均普遍表达。这些数据表明振荡素是一种在整个进化过程中保守的管家基因，并不支持振荡素是负责钙振荡的精子特异性因子这一观点。
