Galdiero F
Zentralbl Bakteriol Orig A. 1975;230(2):203-9.
Cytoplasmic membrane of E. coli is degraded within 20 min following infection with Bdellovibrio. 50% of cellular 42-K is lost during the first 10 min. The cytoplasmic membrane, 20 min after infection, centrifugated on a sucrose gradient produces a wide band containing the main enzyme activities (succinic dehydrogenase and lactic dehydrogenase) bound to the membrane. The incorporation into Bdellovibrio of labelled host cell constituents during intracellular growth has been studied at successive intervals during the development cycle in diluted nutrient broth (about 3 hrs). The cells were broken in a Sorvall-Ribi cell fractionator and the Bdellovibrios separated by centrifugation on sucrose gradient. Polysaccharides, proteins and lipids of Bdellovibrio derive from the utilization of components of the host cells and not from the utilization of the components present in the medium. The incorporation of precursors into polysaccharides and proteins shows the same exponential pattern.
感染蛭弧菌后，大肠杆菌的细胞质膜在20分钟内被降解。在最初的10分钟内，细胞中50%的42-K蛋白丢失。感染20分钟后，将细胞质膜在蔗糖梯度上离心，会产生一条宽带，其中包含与膜结合的主要酶活性（琥珀酸脱氢酶和乳酸脱氢酶）。在稀释的营养肉汤（约3小时）中，研究了蛭弧菌在细胞内生长期间连续间隔内标记宿主细胞成分的掺入情况。细胞在索瓦尔德-里比细胞分级器中破碎，然后通过在蔗糖梯度上离心分离蛭弧菌。蛭弧菌的多糖、蛋白质和脂质来自宿主细胞成分的利用，而非培养基中成分的利用。前体掺入多糖和蛋白质呈现相同的指数模式。
