Saito K, Naito I, Seki T, Oohashi T, Kimura E, Momota R, Kishiro Y, Sado Y, Yoshioka H, Ninomiya Y
Department of Molecular Biology and Biochemistry, Okayama University Medical School, Shikata-cho, Okayama 700-8558, Japan.
J Biochem. 2000 Sep;128(3):427-34. doi: 10.1093/oxfordjournals.jbchem.a022770.
We first completed the primary structure of the mouse alpha5(IV) and alpha6(IV) chains, from which synthetic peptides were produced and a chain-specific monoclonal antibodies were raised. Expression of collagen IV genes in various basement membranes underlying specific organ epithelia was analyzed by immunohistochemical staining using these monoclonal antibodies and other antibodies from human and bovine sequences. It was possible to predict the presence of the three collagen IV molecules: alpha1(IV) alpha2(IV), alpha3(IV)alpha4(IV)alpha5(IV), and alpha5(IV)alpha6(IV). In skin basement membrane two of the three forms, alpha1(IV)alpha2(IV) and alpha5(IV)alpha6(IV), were detected. The alpha3(IV)alpha4(IV)alpha5(IV) molecule was observed as the major form in glomerulus, alveolus, and choroid plexus, where basement membranes function as filtering units. The molecular form alpha5(IV)alpha6(IV) was present in basement membranes in tubular organs such as the epididymis, where the tubes need to expand in diameter. Thus, the distribution of the basement membranes with different molecular composition is consistent with tissue-specific function.
我们首先完成了小鼠α5(IV)和α6(IV)链的一级结构，由此制备了合成肽并产生了链特异性单克隆抗体。使用这些单克隆抗体以及来自人源和牛源序列的其他抗体，通过免疫组织化学染色分析了IV型胶原蛋白基因在特定器官上皮下各种基底膜中的表达。有可能预测三种IV型胶原蛋白分子的存在：[α1(IV)]2α2(IV)、α3(IV)α4(IV)α5(IV)和[α5(IV)]2α6(IV)。在皮肤基底膜中检测到了三种形式中的两种，即[α1(IV)]2α2(IV)和[α5(IV)]2α6(IV)。α3(IV)α4(IV)α5(IV)分子在肾小球、肺泡和脉络丛中作为主要形式被观察到，在这些部位基底膜起到过滤单位的作用。分子形式[α5(IV)]2α6(IV)存在于诸如附睾等管状器官的基底膜中，在这些部位管子需要扩大直径。因此，具有不同分子组成的基底膜的分布与组织特异性功能是一致的。
