Fukumori T, Akari H, Yoshida A, Fujita M, Koyama A H, Kagawa S, Adachi A
Department of Virology, The University of Tokushima School of Medicine, 770-8503, Tokushima, Japan.
Microbes Infect. 2000 Jul;2(9):1011-7. doi: 10.1016/s1286-4579(00)01255-7.
Biological effects of HIV-1 Vpr on CD4(+) cells were studied by an infection system. High-titered HIV-1 stocks pseudotyped with vesicular stomatitis virus G protein were prepared and used to inoculate into CD4(+ )T cells at high multiplicity of infection. Both cell- and virion-associated Vpr were demonstrated to arrest the cell cycle at the G2/M phase, and to induce cell apoptosis. Of note, morphologically apoptotic cells were shown to be arrested at the G2/M stage. No appreciable effect of Vpr on the anti-Fas antibody-mediated apoptosis was observed in this system.
通过感染系统研究了HIV-1 Vpr对CD4(+)细胞的生物学效应。制备了用水泡性口炎病毒G蛋白假型化的高滴度HIV-1毒株，并以高感染复数接种到CD4(+)T细胞中。细胞相关和病毒体相关的Vpr均被证明能使细胞周期停滞在G2/M期，并诱导细胞凋亡。值得注意的是，形态学上的凋亡细胞显示停滞在G2/M期。在该系统中未观察到Vpr对抗Fas抗体介导的凋亡有明显影响。
