Goffeau A
Unité de Biochimie Physiologique, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
FEBS Lett. 2000 Aug 25;480(1):37-41. doi: 10.1016/s0014-5793(00)01775-0.
Four years after disclosure of the full yeast genome sequence, a series of resources including tens of thousands of mutant strains, plasmids bearing isolated genes and disruption cassettes are becoming publicly available. Deletions of each of the 6,000 putative yeast genes are being screened systematically for dozens of phenotypic traits. In addition, new global approaches such as DNA hybridization arrays, quantitative proteomics and two-hybrid interactions are being steadily improved. They progressively build up an immense computation network of billions of data points which will, within the next decade, characterize all molecular interactions occurring in a simple eukaryotic cell. In this process of acquisition of new basic knowledge, an international community of over 1,000 laboratories cooperates with a remarkable willingness to share projects and results.
在酵母全基因组序列公布四年后，一系列资源正逐渐公开，包括数以万计的突变菌株、携带分离基因的质粒和基因敲除盒。对6000个推定的酵母基因中的每一个进行缺失突变，并针对数十种表型特征进行系统筛选。此外，诸如DNA杂交阵列、定量蛋白质组学和双杂交相互作用等新的全局分析方法也在不断改进。它们逐步构建起一个由数十亿个数据点组成的庞大计算网络，在未来十年内，该网络将描绘出简单真核细胞中发生的所有分子相互作用。在获取这些新基础知识的过程中，由1000多个实验室组成的国际团队以极高的热情开展合作，分享项目和研究成果。
