Gont L, Lough J
Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA.
Anat Rec. 2000 Sep 1;260(1):102-5. doi: 10.1002/1097-0185(20000901)260:1<102::AID-AR110>3.0.CO;2-1.
Whole mount in situ hybridization was performed to determine the expression patterns of cSmad1 and cSmad5 during gastrulation stages (Hamburger-Hamilton 2-6) of chicken embryogenesis. It was revealed that cSmads 1 and 5 mRNAs are prominently expressed in the developing primitive streak. As determined from cross-sectional analysis of stage 4 embryos, most transcripts were associated with the ingressing mesoderm. However, whereas cSmad5 was expressed throughout the streak at all stages examined, the strongest expression of cSmad1 was confined to the posterior half of the streak.
进行了全胚胎原位杂交，以确定鸡胚胎发育原肠胚形成阶段（汉伯格-汉密尔顿2-6期）cSmad1和cSmad5的表达模式。结果显示，cSmad1和cSmad5 mRNA在发育中的原条中显著表达。从4期胚胎的横断面分析可知，大多数转录本与内陷的中胚层相关。然而，在所有检测阶段，cSmad5在整个原条中均有表达，而cSmad1的最强表达局限于原条后半部。
