Colin T, Bories A, Moulin G
Laboratoire de Microbiologie Industrielle et Génétique des Micro-organismes, INRA, Gruissan, France.
Appl Microbiol Biotechnol. 2000 Aug;54(2):201-5. doi: 10.1007/s002530000365.
1,3-Propanediol inhibition during glycerol fermentation to 1,3-propanediol by Clostridium butyricum CNCM 1211 has been studied. The initial concentration of the 1,3-propanediol affected the growth of the bacterium more than the glycerol fermentation. mu(max) was inversely proportional to the initial concentration of 1,3-propanediol (0-65 g l(-1)). For glycerol at 20 g l(-1), the growth and fermentation were completely stopped at an initial 1,3-propanediol concentration of 65 g l(-1). However, for an initial 1,3-propanediol concentration of 50 g l(-1) and glycerol at 70 g l(-1), the final concentration (initial and produced) of 1,3-propanediol reached 83.7 g l(-1)(1.1 M), with complete consumption of the glycerol. Therefore, during the fermentation, the strain tolerated a 1,3-propanediol concentration higher than the initial inhibitory concentration (65 g l(-1)). The addition of 1,2-propanediol or 2,3-butanediol (50 g l(-1)) in the presence of glycerol (50-100 g l(-1)), showed that 2-diols reduced the mu(max) in a similar way to 1,3-propanediol. The measurement of the osmotic pressure of glycerol solutions, diols and diol/glycerol mixtures did not indicate any differences between these compounds. The hypothesis of diol inhibition was discussed. Taking into account the strain tolerance of highly concentrated 1,3-propanediol during fermentation, the fermentation processes for optimising production were considered.
已对丁酸梭菌CNCM 1211在甘油发酵生成1,3 - 丙二醇过程中1,3 - 丙二醇抑制作用进行了研究。1,3 - 丙二醇的初始浓度对细菌生长的影响大于对甘油发酵的影响。最大比生长速率（μmax）与1,3 - 丙二醇的初始浓度（0 - 65 g l⁻¹）成反比。对于20 g l⁻¹的甘油，当初始1,3 - 丙二醇浓度为65 g l⁻¹时，生长和发酵完全停止。然而，对于初始1,3 - 丙二醇浓度为50 g l⁻¹和70 g l⁻¹的甘油，1,3 - 丙二醇的最终浓度（初始和生成的）达到83.7 g l⁻¹（1.1 M），甘油被完全消耗。因此，在发酵过程中，该菌株耐受的1,3 - 丙二醇浓度高于初始抑制浓度（65 g l⁻¹）。在甘油（50 - 100 g l⁻¹）存在下添加1,2 - 丙二醇或2,3 - 丁二醇（50 g l⁻¹），结果表明二元醇以与1,3 - 丙二醇类似的方式降低了μmax。对甘油溶液、二元醇以及二元醇/甘油混合物的渗透压测量未显示这些化合物之间存在任何差异。讨论了二元醇抑制的假设。考虑到发酵过程中该菌株对高浓度1,3 - 丙二醇的耐受性，对优化生产的发酵工艺进行了考量。
