Park B Y, Good R A
Birth Defects Orig Artic Ser. 1975;11(1):10-1.
A new micromethod for evaluating lymphocyte responses to phytohemagglutinin (PHA) has been developed and used for the survey of T-cell function in human peripheral blood. The results of lymphocyte responses to PHA stimulation in whole blood culture were expressed in terms of unit volume of blood (50 mul). This new method has eliminated the major uncontrollable factors of conventional methods, and appears to be more correlated with the cell-mediated immunity in vivo.
一种用于评估淋巴细胞对植物血凝素(PHA)反应的新微量方法已被开发出来,并用于检测人类外周血中的T细胞功能。全血培养中淋巴细胞对PHA刺激的反应结果以单位体积血液(50微升)来表示。这种新方法消除了传统方法中主要的不可控因素,并且似乎与体内细胞介导的免疫反应更相关。