Tworzydło M, Laczka M, Rajwa B, Dobrucki J, Turyna B
Department of Animal Biochemistry, Institute of Molecular Biology, Jagiellonian University, Cracow, Poland.
Folia Histochem Cytobiol. 2000;38(3):133-41.
The aim of this work has been to test the biocompatibility of four bioactive, gel derived glass-ceramic materials of CaO-PO2-SiO2 system, modified by addition of boron, aluminum and magnesium compounds. We have examined the growth, collagen synthesis, adhesion and morphology of NRK rat fibroblasts cultured in direct and indirect contact with biomaterials. The growth of cells cultures has been quantified by two methods: [3H]thymidine incorporation and direct counting of cells. The level of collagen synthesis has been used as a parameter describing metabolic activity of cells. Cellular morphology has been assessed following 24 h and 4 days of culturing cells on biomaterials by using SEM and confocal microscopy, respectively. Additionally, in order to obtain information about the attachment of cells to substratum the presence of focal contacts has been examined. The results of all the experiments have demonstrated that none of the materials under study significantly altered cellular functions that were tested. This indicates that additions of MgO, Al2O3 and B2O3 have not induced cytotoxicity of the materials under study. This qualifies them for further in vivo experiments.
这项工作的目的是测试四种生物活性的、凝胶衍生的CaO-PO₂-SiO₂系统玻璃陶瓷材料的生物相容性，这些材料通过添加硼、铝和镁化合物进行了改性。我们研究了与生物材料直接和间接接触培养的NRK大鼠成纤维细胞的生长、胶原蛋白合成、粘附和形态。细胞培养物的生长通过两种方法进行定量：[³H]胸腺嘧啶核苷掺入法和细胞直接计数法。胶原蛋白合成水平已被用作描述细胞代谢活性的参数。分别使用扫描电子显微镜（SEM）和共聚焦显微镜，在生物材料上培养细胞24小时和4天后评估细胞形态。此外，为了获得有关细胞附着于基质的信息，还检查了粘着斑的存在情况。所有实验结果表明，所研究的材料均未显著改变所测试的细胞功能。这表明添加MgO、Al₂O₃和B₂O₃并未诱导所研究材料的细胞毒性。这使它们有资格进行进一步的体内实验。
