Zhang H, Emmons S W
Department of Molecular Genetics, Albert Einstein College of Medicine, The Bronx, New York 10461, USA.
Genes Dev. 2000 Sep 1;14(17):2161-72. doi: 10.1101/gad.814700.
The Caenorhabditis elegans caudal homolog, pal-1, is required for neurogenesis in the male tail. We show that expression of pal-1 in the postembryonic neuroblast cell V6 can be initiated by two alternate pathways. One pathway, acting in wild type, requires a regulatory element in the fifth pal-1 intron. The other pathway, independent of this element, is normally repressed by the newly identified gene sop-1, which encodes a homolog of the mammalian Mediator complex protein TRAP230. In sop-1 mutants, pal-1 is activated by a pathway that is stimulated by bar-1/beta-catenin, a component of the Wnt signal transduction pathway. The results support a physiological role of the Mediator complex in conveying regulatory signals to the transcriptional apparatus.
秀丽隐杆线虫的尾部同源物pal-1是雄性尾部神经发生所必需的。我们发现，胚胎后神经母细胞V6中pal-1的表达可由两条替代途径启动。一条途径在野生型中起作用，需要pal-1第五个内含子中的一个调控元件。另一条途径独立于该元件，通常被新鉴定的基因sop-1抑制，sop-1编码哺乳动物中介体复合物蛋白TRAP230的同源物。在sop-1突变体中，pal-1由bar-1/β-连环蛋白（Wnt信号转导途径的一个组分）刺激的途径激活。这些结果支持了中介体复合物在将调控信号传递给转录装置中的生理作用。
