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母性行为会刺激哺乳期大鼠体内含雌激素受体α的神经元中的c-fos活性。

Maternal behavior stimulates c-fos activity within estrogen receptor alpha-containing neurons in lactating rats.

作者信息

Lonstein J S, Gréco B, De Vries G J, Stern J M, Blaustein J D

机构信息

Center for Neuroendocrine Studies, University of Massachusetts, Amherst, Mass., 01003-7720, USA.

出版信息

Neuroendocrinology. 2000 Aug;72(2):91-101. doi: 10.1159/000054576.

Abstract

Estradiol and other hormones are thought to be critical for the onset, but not maintenance, of maternal behavior in rats. Maternal behavior is instead maintained postpartum by tactile stimulation that dams receive during interactions with pups, and many neural sites implicated in the control of maternal behavior show elevated c-fos activity in response to this stimulation. Many of these sites also contain neurons that express the alpha subtype of the estrogen receptor (ERalpha). Because of possible interactions between tactile stimulation from pups, c-fos, and ERalpha in the lactating rat brain, we determined if populations of cells that show increased c-fos activity after maternal behavior in lactating rats also contain ERalpha. Dams were separated from their pups for 48 h beginning on day 5 postpartum. On day 7 postpartum, experimental dams were reunited with pups and mother-litter interactions were observed for 60 min. Control dams received no pup stimulation. Subjects were sacrificed 60 min later and brain sections were double immunolabeled for the Fos and ERalpha proteins. As expected, the number of ERalpha-immunoreactive (ERalpha-ir) neurons did not differ between the two groups in the eight areas analyzed (lateral region of the lateral septum, posterodorsal medial amygdala, dorsal and ventral medial preoptic area, dorsal and ventral bed nucleus of the stria terminalis, lateral habenula, and ventrolateral caudal periaqueductal gray). Consistent with previous reports, maternal dams had 2- to 7-fold more Fos-immunoreactive (Fos-ir) neurons in these sites compared with nonstimulated controls. Maternal dams had significantly more Fos-ir neurons that also contained ERalpha-ir in all sites, with the greatest increases in the ventral medial preoptic area, lateral habenula, and ventral bed nucleus of the stria terminalis. Between approximately 25 and 45% of the Fos-ir cells in the sites examined also expressed ERalpha. Thus, a substantial number of neurons that are genomically activated during maternal behavior contain ERalpha, raising the possibility that the postpartum display of maternal behavior can be influenced by ERalpha activity.

摘要

雌二醇和其他激素被认为对大鼠母性行为的启动至关重要,但对其维持并非如此。相反,母性行为在产后通过母鼠与幼崽互动时所接受的触觉刺激得以维持,并且许多与母性行为控制相关的神经部位在对这种刺激的反应中表现出c-fos活性升高。这些部位中的许多也含有表达雌激素受体α亚型(ERα)的神经元。由于哺乳期大鼠大脑中来自幼崽的触觉刺激、c-fos和ERα之间可能存在相互作用,我们确定了在哺乳期大鼠母性行为后c-fos活性增加的细胞群体是否也含有ERα。从产后第5天开始,将母鼠与其幼崽分离48小时。在产后第7天,将实验母鼠与幼崽重新团聚,并观察母鼠与幼崽的互动60分钟。对照母鼠未接受幼崽刺激。60分钟后处死实验对象,并对脑切片进行Fos和ERα蛋白的双重免疫标记。正如预期的那样,在分析的八个区域(外侧隔区外侧部、杏仁核后内侧背侧、视前内侧核背侧和腹侧、终纹床核背侧和腹侧、外侧缰核以及导水管周围灰质尾侧腹外侧)中,两组之间ERα免疫反应性(ERα-ir)神经元的数量没有差异。与先前的报道一致,与未受刺激的对照相比,母性母鼠在这些部位的Fos免疫反应性(Fos-ir)神经元多2至7倍。在所有部位中母性母鼠含有ERα-ir的Fos-ir神经元明显更多,在视前内侧核腹侧、外侧缰核和终纹床核腹侧增加最为显著。在所检查部位中,约25%至45%的Fos-ir细胞也表达ERα。因此,在母性行为期间基因组被激活的大量神经元含有ERα,这增加了产后母性行为表现可能受ERα活性影响的可能性。

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