Wieser R, Volz A, Schnittger S, Jäger U, Grüner H, Meran J G, Wimmer K, Ziegler A, Fonatsch C
Institut für Medizinische Biologie der Universität Wien, Austria.
Br J Haematol. 2000 Aug;110(2):343-50. doi: 10.1046/j.1365-2141.2000.02192.x.
Chromosome aberrations affecting band 3q21 are associated with a particularly poor prognosis in patients with acute myeloid leukaemia. To facilitate the molecular characterization of such rearrangements, we established a PAC contig covering the relevant genomic region. Using these PACs as probes in fluorescence in situ hybridization (FISH) experiments, we showed that a number of 3q21 breakpoints in patient samples map to a previously defined 'breakpoint cluster region'. Others, however, are located at varying distances centromeric of it. These results have important implications in the search for genes affected by 3q21 rearrangements.
