用¹³¹I标记的奥硝唑检测炎症

Detecting inflammation with 131I-labeled ornidazole.

作者信息

Asikoglu M, Yurt F, Cagliyan O, Unak P, Ozkilic H

机构信息

Faculty of Pharmacy, Department of Radiopharmacy, Ege University, Bornova, Izmir, Turkey.

出版信息

Appl Radiat Isot. 2000 Sep;53(3):411-3. doi: 10.1016/s0969-8043(99)00150-5.

DOI:10.1016/s0969-8043(99)00150-5

PMID:10972145

Abstract

The aim of this study was to demonstrate the accumulation of 131I-labeled ornidazole (131I-ORN) in experimental abscesses. 131I-ORN was prepared by electrophilic radioiodination of ORN, using radioiodide in the presence of Iodogen. An in vivo inflammation model was prepared by intramuscular injection of turpentine into the thigh of rabbits. Four days later 131I-ORN was intravenously administered to rabbits. Serial scintigrams were performed at different periods, using a Sophy DX Gamma Camera. 131I-ORN was visualized at 10 min after injection. 131I-ORN was also administered intraperitoneally to rats with turpentine-induced inflammation, for quantitative biodistribution studies. Counts of selected tissues were taken by a NaI(Tl) scintillation detector (gamma counter) after rats were decapitated. The target-to-non-target muscle ratios were 2.5, 2.6, 2.9 and 1.9 at 1, 3, 5 and 24 h, respectively.

摘要

本研究的目的是证明131I标记的奥硝唑（131I-ORN）在实验性脓肿中的蓄积情况。131I-ORN通过在碘代胆甾醇存在下用放射性碘对ORN进行亲电放射性碘化来制备。通过向兔大腿肌肉注射松节油制备体内炎症模型。四天后，将131I-ORN静脉注射给兔。使用Sophy DX伽马相机在不同时间段进行连续闪烁扫描。注射后10分钟可观察到131I-ORN。还将131I-ORN腹腔注射给患有松节油诱导炎症的大鼠，用于定量生物分布研究。大鼠断头后，用NaI（Tl）闪烁探测器（伽马计数器）对选定组织进行计数。在1、3、5和24小时时，靶组织与非靶肌肉的比值分别为2.5、2.6、2.9和1.9。