半乳糖结合蛋白的“隐藏配体”

The "hidden ligand" of the galactose-binding protein.

作者信息

Silhavy T J, Boos W

出版信息

Eur J Biochem. 1975 May;54(1):163-7. doi: 10.1111/j.1432-1033.1975.tb04125.x.

DOI:10.1111/j.1432-1033.1975.tb04125.x

Abstract

Following tryptophan fluorescence of the galactose-binding during dissociation of the ligand it has been found that glucose dissociates with a half life of less than 5 s. Similarly, fast dissociation was also observed by following release of radioactively labelled glucose from Sepharose-coupled galactose-binding protein upon dilution. Accordingly, a previous claim that the galactose-binding protein contains glucose as a non-dissociable "hidden ligand" [G. Richarme and A. Kepes (1974) Eur. J. Biochem. 45, 127-133] has to be reinterpreted

摘要

在配体解离过程中对半乳糖结合蛋白的色氨酸荧光进行跟踪后发现，葡萄糖解离的半衰期小于5秒。同样，通过稀释从琼脂糖偶联的半乳糖结合蛋白中释放放射性标记的葡萄糖，也观察到了快速解离。因此，先前关于半乳糖结合蛋白含有葡萄糖作为不可解离的“隐藏配体”的说法[G. Richarme和A. Kepes（1974年）《欧洲生物化学杂志》45卷，第127 - 133页]必须重新解释。