Inada M, Glass G B
Gastroenterology. 1975 Aug;69(2):396-408.
In order to determine the possible effects of the circulating intrinsic factor antibodies (IFA) on gastric morphology and secretory function, four groups of 12 rats each received intravenous injection daily for 8 to 12 weeks of immunoglobulin G (IgG) fractions separated on DEAE-cellulose columns from various sources: (1) sera of patients with pernicious anemia, containing both IFA and parietal cell antibodies (PCA), (2) sera from patients with atrophic gastritis, containing parietal cell antibody only, and (3) and (4) sera of rabbits immunized with semipurified human or rat intrinsic factor (IF). In addition three control groups of 12 rats each received intravenous injections daily for 8 to 12 weeks of either (5) saline or (6) and (7) IgG processed from human or rabbit normal sera. Still another group of 12 rats (8) did not receive any injections whatsoever for the same duration of time. One-third of the rats were intubated biweekly after histamine stimulation and the hourly outputs of CHl pepsin, and IF were determined. At conclusion of the experiments, rats were killed, the mucosal surface and thickness of the mucosa were measured, and parietal cell and peptic cell masses were counted. The control groups showed either progressive growth of the cellular mass in gastric mucosa and increase of the HCl, pepsin, and IF outputs, or no significant changes. In contrast, rats injected with IgG containing IFA to human or rat IF showed a statistically significant thinning of the gastric mucosa, reduction of peptic cells, which are known to secret IF in this species, and corresponding decreases in the ouputs of pepsin and IF. These became reduced by about 50% from initial values, and by 62 or 75%, respectively, when compared to rats injected with normal human or rabbit IgG's. In rats injected with IgG's from pernicious anemia sera, which contained both IFA and PCA, the outputs of IF, pepsin, and HCl decreased signigicantly, as well as the peptic and parietal cell masses. The rats injected with PCA only demonstrated thinning of the gastric mucosa, reduction of parietal cell mass, and a significant decrease of the HCl output. These findings imply an active role of the circulating gastric.
为了确定循环中的内因子抗体（IFA）对胃形态和分泌功能可能产生的影响，将四组每组12只大鼠，每天静脉注射从不同来源的DEAE - 纤维素柱上分离得到的免疫球蛋白G（IgG）组分，持续8至12周：（1）恶性贫血患者的血清，含有IFA和壁细胞抗体（PCA）；（2）萎缩性胃炎患者的血清，仅含有壁细胞抗体；以及（3）和（4）用半纯化的人或大鼠内因子（IF）免疫的兔子的血清。另外，三个对照组每组12只大鼠，每天静脉注射持续8至12周，注射的分别是（5）生理盐水或（6）和（7）从人或兔正常血清中提取的IgG。还有另一组12只大鼠（8）在相同时间段内未接受任何注射。三分之一的大鼠在组胺刺激后每两周插管一次，测定每小时的胃酸、胃蛋白酶和内因子的分泌量。实验结束时，处死大鼠，测量胃黏膜表面和厚度，并计数壁细胞和主细胞数量。对照组显示胃黏膜细胞团要么逐渐生长，胃酸、胃蛋白酶和内因子分泌量增加，要么无显著变化。相比之下，注射含人或大鼠IF的IFA的IgG的大鼠，胃黏膜在统计学上显著变薄，已知该物种中分泌内因子的主细胞数量减少，胃蛋白酶和内因子分泌量相应降低。这些分泌量与初始值相比减少了约50%，与注射正常人或兔IgG的大鼠相比，分别减少了62%或75%。注射含有IFA和PCA的恶性贫血血清IgG的大鼠，内因子、胃蛋白酶和胃酸的分泌量以及主细胞和壁细胞数量均显著减少。仅注射PCA的大鼠表现出胃黏膜变薄，壁细胞数量减少，胃酸分泌量显著降低。这些发现表明循环中的胃抗体具有积极作用。
