通过液氮冷冻用于检测DEPMPO-超氧自由基的电子顺磁共振（EPR）的改进方法

Improved method for EPR detection of DEPMPO-superoxide radicals by liquid nitrogen freezing.

作者信息

Dambrova M, Baumane L, Kalvinsh I, Wikberg J E

机构信息

Department of Medicinal Chemistry, Latvian Institute of Organic Synthesis, Aizkraukles St. 21, Riga, LV-1006, Latvia.

出版信息

Biochem Biophys Res Commun. 2000 Sep 7;275(3):895-8. doi: 10.1006/bbrc.2000.3387.

DOI:10.1006/bbrc.2000.3387

PMID:10973818

Abstract

5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO) is frequently used as a spin trap for the measurement of superoxide by EPR spectrometry. However, its half life is fairly short in room temperature. We here show that superoxide radicals trapped by DEPMPO can be successfully recorded at -196 degrees C. Moreover, we show that the signal intensity remains unaltered for up to 7 days, when the samples are stored in liquid nitrogen. Our new approach for measurement of superoxide should greatly simplify the studies of this important radical in biological systems.

摘要

5-二乙氧基磷酰基-5-甲基-1-吡咯啉N-氧化物（DEPMPO）经常被用作自旋捕集剂，通过电子顺磁共振光谱法测量超氧化物。然而，它在室温下的半衰期相当短。我们在此表明，被DEPMPO捕获的超氧自由基在-196℃时能够成功记录。此外，我们还表明，当样品储存在液氮中时，信号强度在长达7天的时间内保持不变。我们测量超氧化物的新方法应能极大地简化对生物系统中这种重要自由基的研究。