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由新型氨基反应性DEPMPO自旋捕获剂合成的线粒体-DEPMPO：一种用于检测超氧化物的新型改良捕获剂。

Mito-DEPMPO synthesized from a novel NH2-reactive DEPMPO spin trap: a new and improved trap for the detection of superoxide.

作者信息

Hardy Micael, Chalier Florence, Ouari Olivier, Finet Jean-Pierre, Rockenbauer Antal, Kalyanaraman Balaraman, Tordo Paul

机构信息

SREP, UMR 6517 CNRS et Universités Aix-Marseille 1, 2 et 3, Centre de Saint Jérôme, 13013 Marseille, France.

出版信息

Chem Commun (Camb). 2007 Mar 14(10):1083-5. doi: 10.1039/b616076j. Epub 2007 Jan 2.

DOI:10.1039/b616076j
PMID:17325813
Abstract

Mito-DEPMPO, a new DEPMPO analogue bearing a triphenylphosphonium group, was synthesized via a novel NH2-reactive DEPMPO. The half-life of the Mito-DEPMPO superoxide adduct was estimated to be ca. 40 min. Using Mito-DEPMPO, reactive oxygen species generated in intact mitochondria were detected and characterized by EPR.

摘要

线粒体-DEPMPO是一种带有三苯基鏻基团的新型DEPMPO类似物，它是通过一种新型的NH2反应性DEPMPO合成的。线粒体-DEPMPO超氧化物加合物的半衰期估计约为40分钟。利用线粒体-DEPMPO，通过电子顺磁共振（EPR）对完整线粒体中产生的活性氧进行了检测和表征。

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