有核细胞和血浆DNA双向母婴转运的定量分析。
Quantitative analysis of the bidirectional fetomaternal transfer of nucleated cells and plasma DNA.
作者信息
Lo Y M, Lau T K, Chan L Y, Leung T N, Chang A M
机构信息
Departments of Chemical Pathology and Obstetrics and Gynecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR.
出版信息
Clin Chem. 2000 Sep;46(9):1301-9.
BACKGROUND
Recently, much interest has been generated on the fetomaternal transfer of nucleated cells and plasma DNA. However, there has been no systematic quantitative comparison of these two directions and two modalities of trafficking within the same study population.
METHODS
The fetus-to-mother transfer of nucleated cells and plasma DNA in pregnant women carrying male babies was studied using a real-time quantitative PCR assay for the S:RY gene. For mother-to-fetus transfer, real-time quantitative PCR assays for the insertion/deletion polymorphisms involving the glutathione S:-transferase M1 and angiotensin-converting enzyme genes were used.
RESULTS
Of the 50 informative mother-baby pairs, maternal DNA was detected in the cellular fraction of umbilical cord blood in 24% of cases (12 of 50), at a median fractional concentration of 2.6 x 10(-4) (interquartile range, 1.7 x 10(-4) to 3.6 x 10(-4)). In the plasma fraction of cord blood, maternal DNA was detected in 30% (15 of 50) of cases at a median fractional concentration of 3 x 10(-3) (interquartile range, 1 x 10(-3) to 1.6 x 10(-2)). For the other direction of trafficking, fetus-to-mother transfer of nucleated cells was detected in 26% of cases (13 of 50) at a median fractional concentration of 3.2 x 10(-4) (interquartile range, 0.6 x 10(-4) to 7.6 x 10(-4)). In the plasma fraction, fetal DNA was detected in 100% of maternal plasma (50 of 50) at a median fractional concentration of 3 x 10(-2) (interquartile range, 1.4 x 10(-2) to 5. 3 x 10(-2)).
CONCLUSIONS
This study indicated that significantly more fetal DNA is present in the plasma of pregnant women compared with DNA from the cellular fraction of maternal blood. In addition, maternal DNA was demonstrated in both the cellular and plasma fractions of cord blood after delivery. This study has therefore determined the fundamental quantitative values for the bidirectional fetomaternal cellular and plasma DNA traffic.
背景
最近，有核细胞和血浆DNA的母胎转运引发了广泛关注。然而，在同一研究人群中，尚未对这两种转运方向和两种转运方式进行系统的定量比较。
方法
采用针对S:RY基因的实时定量PCR检测法，研究怀有男婴的孕妇中核细胞和血浆DNA的胎儿向母亲的转运。对于母亲向胎儿的转运，采用针对谷胱甘肽S:-转移酶M1和血管紧张素转换酶基因插入/缺失多态性的实时定量PCR检测法。
结果
在50对有信息价值的母婴对中，24%（50例中的12例）的脐带血细胞部分检测到母体DNA，中位分数浓度为2.6×10⁻⁴（四分位间距，1.7×10⁻⁴至3.6×10⁻⁴）。在脐带血血浆部分，30%（50例中的15例）检测到母体DNA，中位分数浓度为3×10⁻³（四分位间距，1×10⁻³至1.6×10⁻²）。对于另一个转运方向，26%（50例中的13例）检测到有核细胞的胎儿向母亲的转运，中位分数浓度为3.2×10⁻⁴（四分位间距，0.6×10⁻⁴至7.6×10⁻⁴）。在血浆部分，100%的母体血浆（50例中的50例）检测到胎儿DNA，中位分数浓度为3×10⁻²（四分位间距，1.4×10⁻²至5.3×10⁻²）。
结论
本研究表明，与母血有细胞部分的DNA相比，孕妇血浆中存在的胎儿DNA明显更多。此外，分娩后脐带血的细胞和血浆部分均检测到母体DNA。因此，本研究确定了双向母胎细胞和血浆DNA转运的基本定量值。
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