用亚精胺或二价阳离子替代镁离子对核糖体的影响。

Effects on ribosomes of the substitution of spermidine or divalent cations for magnesium ions.

作者信息

Igarashi K, Sugawara K, Hirose S

出版信息

J Biochem. 1975 Apr;77(4):753-9. doi: 10.1093/oxfordjournals.jbchem.a130779.

DOI:10.1093/oxfordjournals.jbchem.a130779

Abstract

The inactivation of rat liver ribosomes (polysomes) resulting from the replacement of bound magnesium by spermidine has been studied using a dialysis method. Inactivation of the ribosomes (polysomes) occurred when more than 40% of the Mg2+ originally bound to ribosomes in the presence of 2 mM Mg2+ was replaced by spermidine. The polypeptide-synthesizing activity of polysomes was retained partially when Mg2+ was replaced by Mn2+ by dialysis. E. coli 30S ribosomal subunits were more resistant to inactivation due to the replacement of Mg2+ by spermidine than the 50S subunits.

摘要

利用透析法研究了亚精胺取代结合态镁导致大鼠肝脏核糖体（多核糖体）失活的情况。当在2 mM镁存在下，原本结合在核糖体上的Mg2+超过40%被亚精胺取代时，核糖体（多核糖体）就会失活。通过透析用Mn2+取代Mg2+时，多核糖体的多肽合成活性部分得以保留。与50S亚基相比，大肠杆菌30S核糖体亚基对因亚精胺取代Mg2+而导致的失活更具抗性。