Bauer M K, Harding J E, Breier B H, Gluckman P D
Research Centre for Developmental Medicine and Biology, School of Medicine, Private Bag 92019, Auckland, New Zealand.
J Endocrinol. 2000 Sep;166(3):591-7. doi: 10.1677/joe.0.1660591.
The role of GH in the regulation of fetal growth and metabolism in late gestation is not well defined. The aim of this study was to determine the effects of exogenous GH infusion on fetal growth and feto-placental metabolism in the normally growing late-gestation fetal sheep. Eleven fetuses received pulsatile GH infusion (3.5 mg/day) for 10 days while 12 control fetuses received vehicle. The GH infusion was given as a continuous infusion (2.5 mg/day) plus an additional pulsatile component (30 pulses equivalent to 1 mg/day) designed to mimic the natural pattern of GH secretion. Fetal GH infusion raised the circulating fetal concentrations of GH threefold, but did not change fetal concentrations of IGF-I, IGF-binding protein-3, insulin or ovine placental lactogen. GH-treated fetuses had blood urea concentrations 15% lower than controls (P<0.05) and glucose uptake 18% lower per kg fetal weig! ht (P=0.06). There were no other differences attributable to fetal GH infusion in feto-placental metabolism, placental function or placental blood flow. GH-treated fetuses were larger than controls at postmortem (weight+13%, P<0.01; girth+5%, P<0.01; crown-rump length+3%, P<0.05). However, there were no differences between groups in measures of fetal growth (increment in chest girth and hindlimb length). GH-treated fetuses had heavier mothers and when maternal weight was included as a covariate in the analysis, there was no significant difference between treatment groups that could be attributed to GH treatment. GH infusion to normal fetal sheep does not appear to have a significant effect on feto-placental metabolism or fetal growth.
生长激素（GH）在妊娠晚期对胎儿生长和代谢的调节作用尚未明确。本研究的目的是确定外源性GH输注对正常生长的妊娠晚期胎羊胎儿生长和胎儿-胎盘代谢的影响。11只胎儿接受脉冲式GH输注（3.5毫克/天），持续10天，而12只对照胎儿接受赋形剂。GH输注采用持续输注（2.5毫克/天）加额外的脉冲成分（30次脉冲，相当于1毫克/天），以模拟GH分泌的自然模式。胎儿GH输注使胎儿循环中的GH浓度提高了三倍，但未改变胎儿胰岛素样生长因子-I（IGF-I）、IGF结合蛋白-3、胰岛素或羊胎盘催乳素的浓度。接受GH治疗的胎儿血尿素浓度比对照组低15%（P<0.05），每千克胎儿体重的葡萄糖摄取量低18%（P=0.06）。在胎儿-胎盘代谢、胎盘功能或胎盘血流量方面，没有其他可归因于胎儿GH输注的差异。接受GH治疗的胎儿在死后比对照组更大（体重增加13%，P<0.01；腹围增加5%，P<0.01；顶臀长度增加3%，P<0.05）。然而，两组在胎儿生长指标（胸围和后肢长度的增加）上没有差异。接受GH治疗的胎儿的母亲体重更重，当将母亲体重作为协变量纳入分析时，治疗组之间没有可归因于GH治疗的显著差异。向正常胎羊输注GH似乎对胎儿-胎盘代谢或胎儿生长没有显著影响。
