Durkin H G, Waksman B H
J Immunol. 1975 Jul;115(1):170-6.
Sensitized T cells in inguinal lymph nodes (LN) of rats immunized with ovalbumin and adjuvant can be separated on BSA density gradients from T cells responding to Con A and PHA. Further, these OA-sensitized T cells are separable from B memory cells responsive to OA. On days 9 and 17 after sensitization, OA-reactive cells peak and are found in enriched numbers in the lighter bands A + B (B-band), and C, respectively whereas Con A and PHA reactive cells, on both days, are concentrated in bands C and D, respectively. On day 9 at least 20 to 30% of B-band cells can reinitiate DNA synthesis and produce high concentrations of lymphotoxin in response to OA in culture, but show only a marginal blastogenic response to Con A or PHA and cannot produce lymphotoxin in response to PHA. These cells are defined as OA-sensitized T cells because a) treatment with rat isoantiserum specific for T lymphocytes plus complement abolished their ability to incorporate 3H-thymidine in response to OA and Con A or PHA; b) membrane-bound immunoglobulin could not detected with indirect immunofluorescence staining; c) histologic studies demonstrate massive hypertrophy of thymus-dependent paracortical areas and absence of germinal centers in inguinal LN at this time, and d) they produce lymphotoxin after challenge with OA, but not with PHA, whereas cells of the other bands produce lymphotoxin in response to PHA, but not to OA. Addition of purified macrophages to the various cultures did not affect the levels of response observed. Day 17 C-band cells are regarded as B-memory cells because treatment with T-specific antiserum plus complement enhanced their response to OA, many germinal centers now are present in inguinal LN, and a significant proportion of cells of bands B and C, negative on day 9, show positive immunofluorescence staining at this time.
用卵清蛋白和佐剂免疫的大鼠腹股沟淋巴结（LN）中的致敏T细胞，可以在牛血清白蛋白密度梯度上与对刀豆球蛋白A（Con A）和植物血凝素（PHA）作出反应的T细胞分离。此外，这些卵清蛋白致敏的T细胞可与对卵清蛋白作出反应的B记忆细胞分离。致敏后第9天和第17天，对卵清蛋白有反应的细胞达到峰值，分别在较轻的A + B带（B带）和C带中数量富集，而在这两天，对Con A和PHA有反应的细胞分别集中在C带和D带。在第9天，至少20%至30%的B带细胞能够重新启动DNA合成，并在培养中对卵清蛋白产生高浓度的淋巴毒素，但对Con A或PHA仅表现出微弱的增殖反应，且对PHA不产生淋巴毒素。这些细胞被定义为卵清蛋白致敏的T细胞，原因如下：a）用针对T淋巴细胞的大鼠同种抗血清加补体处理后，它们对卵清蛋白以及Con A或PHA摄取3H-胸腺嘧啶核苷的能力被消除；b）用间接免疫荧光染色未检测到膜结合免疫球蛋白；c）组织学研究表明，此时腹股沟淋巴结中胸腺依赖的副皮质区大量肥大且无生发中心；d）用卵清蛋白攻击后它们产生淋巴毒素，但用PHA攻击则不产生，而其他带的细胞对PHA产生淋巴毒素，但对卵清蛋白不产生。向各种培养物中添加纯化的巨噬细胞不影响观察到的反应水平。第17天的C带细胞被视为B记忆细胞，因为用T特异性抗血清加补体处理增强了它们对卵清蛋白的反应，此时腹股沟淋巴结中有许多生发中心，并且在第9天呈阴性的B带和C带细胞中有相当一部分此时显示出阳性免疫荧光染色。
