Gershanovich V N, Kukanova A Ia, Galushkina Z M, Stepanov A I
Bioengineering Center, Russian Academy of Sciences, Moscow.
Mol Gen Mikrobiol Virusol. 2000(3):3-7.
Unlike its predecessors B. subtilis rosR and 41, riboflavin producing B. subtilis 24 strain does not utilize pentose and gluconate and poorly assimilates glucose. Simultaneous addition of glutamic and shikimic acid restored its capacity to grow and produce riboflavin in medium with pentose and gluconate. This strain lacks the activity of transketolase, the key enzyme of the pentose phosphate cycle, and possesses normal ribulose-5-phosphate-epimerase and glucose phosphate isomerase activities. Like enterobacteria, B. subtilis has two different transport systems for glucose and mannose. The data are discussed from the viewpoint of increasing riboflavin production by transketolase mutants. Probable consequences of cell wall and cytoplasmatic membrane damage in B. subtilis with this mutation are discussed.
与枯草芽孢杆菌rosR和41不同，产核黄素的枯草芽孢杆菌24菌株不利用戊糖和葡萄糖酸盐，对葡萄糖的同化能力也很差。同时添加谷氨酸和莽草酸可恢复其在含有戊糖和葡萄糖酸盐的培养基中生长和产核黄素的能力。该菌株缺乏磷酸戊糖途径的关键酶转酮醇酶的活性，但核糖-5-磷酸表异构酶和葡萄糖磷酸异构酶活性正常。与肠杆菌一样，枯草芽孢杆菌有两种不同的葡萄糖和甘露糖转运系统。从转酮醇酶突变体提高核黄素产量的角度对数据进行了讨论。还讨论了枯草芽孢杆菌发生这种突变时细胞壁和细胞质膜损伤可能产生的后果。
