Lisak R P, Bealmear B
Department of Neurology, Wayne State University School of Medicine, Detroit Medical Center, MI, USA.
J Peripher Nerv Syst. 1997;2(3):233-43.
We investigated the expression and upregulation of intercellular adhesion molecule-1 (ICAM-1) in cultures of neonatal rat Schwann cells. Unstimulated cells expressed little or no ICAM-1 as determined by binding of monoclonal antibody to rat ICAM-1 using qualitative and quantitative immunofluorescence. ICAM-1 positive cells when present represented a very small minority (<4%) of Schwann cells. Incubation with recombinant rat interferon-gamma, recombinant rat IL-1beta, recombinant rat tumor necrosis factor-alpha, unfractionated rat cytokines but not purified porcine transforming growth factor-beta or recombinant rat IL-2, resulted in upregulation of ICAM-1 on Schwann cells. Different kinetics were noted for ICAM-1 upregulation by the different recombinant and purified cytokines. The upregulation of ICAM-1 on Schwann cells may be important in inflammatory diseases of the peripheral nervous system as well as in myelination and remyelination.
我们研究了新生大鼠雪旺细胞培养物中细胞间黏附分子-1（ICAM-1）的表达及上调情况。通过使用定性和定量免疫荧光法，用单克隆抗体与大鼠ICAM-1结合来测定，未受刺激的细胞表达很少或不表达ICAM-1。当存在ICAM-1阳性细胞时，其仅占雪旺细胞的极少数（<4%）。用重组大鼠干扰素-γ、重组大鼠白细胞介素-1β、重组大鼠肿瘤坏死因子-α、未分级的大鼠细胞因子（但不是纯化的猪转化生长因子-β或重组大鼠白细胞介素-2）孵育，会导致雪旺细胞上ICAM-1上调。不同的重组和纯化细胞因子对ICAM-1上调呈现出不同的动力学。雪旺细胞上ICAM-1的上调在外周神经系统的炎症性疾病以及髓鞘形成和髓鞘再生中可能具有重要意义。
