Sheoran A S, Timoney J F, Holmes M A, Karzenski S S, Crisman M V
Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington 40546-0099, USA.
Am J Vet Res. 2000 Sep;61(9):1099-105. doi: 10.2460/ajvr.2000.61.1099.
To determine concentrations of IgA and IgG subclasses in serum, colostrum, milk, and nasal wash samples of adult horses and foals.
Seven 2-year-old Welsh ponies, 27 adult mixed-breed horses, and 5 Quarter Horse mares and their foals.
Serum was obtained from ponies and adult horses. Colostrum and milk were obtained from mares and serum and nasal wash samples from their foals immediately after parturition and on days 1, 7, 14, 28, 42, and 63. Nasal wash samples were also obtained from 23 adult horses. Concentrations of immunoglobulins were determined by use of inhibition ELISA. To determine transfer of maternal isotypes to foals, concentrations in colostrum and milk were compared with those in foal serum. Serum half-lives of isotypes in foals were also determined.
IgGb was the most abundant isotype in serum and colostrum from adult horses, whereas IgA was the predominant isotype in milk. The major isotype in nasal secretions of adult horses and foals > or = 28 days old was IgA, but IgGa and IgGb were the major isotypes in nasal secretions of foals < or = 14 days old. Serum half lives of IgGa, IgGb, IgG(T), and IgA in foals were 176, 32, 21, and 3.4 days, respectively.
The early immunoglobulin repertoire of neonatal foals comprised IgGa, IgG(T), and IgA; endogenous synthesis of IgGb could not be detected until 63 days after birth. The restricted repertoire of immunoglobulins in foals may influence humoral immune responses to vaccination.
测定成年马和幼驹血清、初乳、乳汁及鼻腔冲洗液样本中IgA和IgG亚类的浓度。
7匹2岁威尔士小马、27匹成年杂种马以及5匹夸特马母马及其幼驹。
从小马和成年马采集血清。在分娩后即刻以及第1、7、14、28、42和63天，从母马采集初乳和乳汁，从其幼驹采集血清和鼻腔冲洗液样本。还从23匹成年马采集鼻腔冲洗液样本。使用抑制ELISA法测定免疫球蛋白的浓度。为确定母源同种型向幼驹的转移情况，将初乳和乳汁中的浓度与幼驹血清中的浓度进行比较。还测定了幼驹同种型的血清半衰期。
IgGb是成年马血清和初乳中含量最丰富的同种型，而IgA是乳汁中的主要同种型。成年马和年龄≥28天的幼驹鼻腔分泌物中的主要同种型是IgA，但年龄≤14天的幼驹鼻腔分泌物中的主要同种型是IgGa和IgGb。幼驹中IgGa、IgGb、IgG(T)和IgA的血清半衰期分别为176天、32天、21天和3.4天。
新生幼驹早期的免疫球蛋白谱包括IgGa、IgG(T)和IgA；出生后63天前未检测到IgGb的内源性合成。幼驹免疫球蛋白谱受限可能会影响对疫苗接种的体液免疫反应。
