Barthel R, Piedrahita J A, McMurray D N, Payeur J, Baca D, Suárez Güemes F, Perumaalla V S, Ficht T A, Templeton J W, Adams L G
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4467, USA.
Am J Vet Res. 2000 Sep;61(9):1140-4. doi: 10.2460/ajvr.2000.61.1140.
To determine necropsy and Mycobacterium bovis culture results in cattle from herds with tuberculosis, the role of the bovine NRAMP1 gene in resistance and susceptibility to infection with M bovis, and the association between magnitude of the tuberculous lesions and various types of M bovis isolates.
61 cattle from herds with tuberculosis in Texas and Mexico.
61 cattle were evaluated by necropsy; 59 had positive and 2 had negative caudal fold tuberculin intradermal test (CFT) results. Thirty-three cattle with positive CFT results were genotyped to evaluate polymorphism of the 3' untranslated region of the bovine NRAMP1 gene, using single-stranded conformational analysis, 9 were resistant to M bovis with no tuberculous lesions and negative M bovis culture results, and 24 were susceptible with tuberculous lesions and positive M bovis culture results. Isolates of M bovis were analyzed by restriction fragment length polymorphism (RFLP) on the basis of IS6110 sequences and direct-repeat fingerprinting patterns.
21 (35.6%; 21/59) cattle with positive CFT results had tuberculous lesions or positive culture results; in addition, 1 of 2 cattle with negative CFT results had tuberculous lesions and positive culture results. Tuberculous lesions were most common in the thorax (35/63; 55.5%) and lymphoid tissues of the head (10/63; 15.9%). Tuberculous lesions varied from 1 to 11/animal; 8 of 21 (38.1%) had solitary lesions. Associations were not found between resistance or susceptibility to infection with M bovis and polymorphism in the NRAMP1 gene or between the magnitude of the lesions and various RFLP types of M bovis isolates.
The NRAMP1 gene does not determine resistance and susceptibility to infection with M bovis in cattle.
确定患有结核病牛群中牛的尸检结果及牛型分枝杆菌培养结果，牛天然抗性相关巨噬蛋白1（NRAMP1）基因在牛对牛型分枝杆菌感染的抗性和易感性中的作用，以及结核病变程度与各种类型牛型分枝杆菌分离株之间的关联。
来自得克萨斯州和墨西哥患有结核病牛群的61头牛。
对61头牛进行尸检评估；59头牛尾褶皮内结核菌素试验（CFT）结果为阳性，2头牛结果为阴性。对33头CFT结果为阳性的牛进行基因分型，以评估牛NRAMP1基因3'非翻译区的多态性，采用单链构象分析，9头牛对牛型分枝杆菌具有抗性，无结核病变且牛型分枝杆菌培养结果为阴性，24头牛易感，有结核病变且牛型分枝杆菌培养结果为阳性。基于插入序列6110（IS6110）序列和直接重复指纹图谱，通过限制性片段长度多态性（RFLP）分析牛型分枝杆菌分离株。
59头CFT结果为阳性的牛中，21头（35.6%；21/59）有结核病变或培养结果为阳性；此外，2头CFT结果为阴性的牛中，有1头有结核病变且培养结果为阳性。结核病变最常见于胸部（35/63；55.5%）和头部淋巴组织（10/63；15.9%）。每头动物的结核病变数量为1至11个；21头中有8头（38.1%）有单个病变。在牛对牛型分枝杆菌感染的抗性或易感性与NRAMP1基因多态性之间，以及病变程度与牛型分枝杆菌分离株的各种RFLP类型之间未发现关联。
NRAMP1基因不能决定牛对牛型分枝杆菌感染的抗性和易感性。
