Bartels H, Gluehmann M, Janell D, Schluenzen F, Tocilj A, Bashan A, Levin I, Hansen H A, Harms J, Kessler M, Pioletti M, Auerbach T, Agmon I, Avila H, Simitsopoulou M, Weinstein S, Peretz M, Bennett W S, Franceschi F, Yonath A
Department of Structural Biology, Weizmann Institute, Rehovot, Israel.
Cell Mol Biol (Noisy-le-grand). 2000 Jul;46(5):871-82.
Within the framework of ribosomal crystallography, the small subunits are being analyzed, using crystals diffracting to 3 A resolution. The medium resolution electron density map of this subunit, obtained by multiple isomorphous replacement, show recognizable morphologies, strikingly similar to the functional active conformer of the small ribosomal subunit. It contains elongated dense features, traceable as RNA chains as well as globular regions into which the structures determined for isolated ribosomal proteins, or other known structural motifs were fitted. To facilitate unbiased map interpretation, metal clusters are being covalently attached either to the surface of the subunits or to DNA oligomers complementary to exposed ribosomal RNA. Two surface cysteines and the 3' end of the 16S ribosomal RNA have been localized. Targeting several additional RNA regions shed light on their relative exposure and confirmed previous studies concerning their functional relevance.
在核糖体晶体学的框架内，正在使用衍射至3埃分辨率的晶体对小亚基进行分析。通过多重同晶置换获得的该亚基的中等分辨率电子密度图显示出可识别的形态，与小核糖体亚基的功能活性构象惊人地相似。它包含细长的致密特征，可追溯为RNA链以及球状区域，已确定的分离核糖体蛋白或其他已知结构基序的结构可拟合到这些球状区域中。为便于进行无偏差的图谱解释，金属簇正被共价连接到亚基表面或与暴露的核糖体RNA互补的DNA寡聚物上。已定位了两个表面半胱氨酸和16S核糖体RNA的3'末端。针对几个额外的RNA区域揭示了它们的相对暴露情况，并证实了先前关于它们功能相关性的研究。
