日本猕猴（食蟹猴）成年和胎儿血红蛋白的氧平衡特征

Oxygen equilibrium characteristics of adult and fetal hemoglobin of Japanese monkey (Macaca fuscata).

作者信息

Takenaka O, Morimoto H

出版信息

Biochim Biophys Acta. 1976 Oct 28;446(2):457-62. doi: 10.1016/0005-2795(76)90011-8.

Abstract

Adult hemoglobin and fetal hemoglobin were obtained from Japanese monkey (Macaca fuscata) and their oxygen equilibrium characteristics were studied. (1) The oxygen affinity of fetal hemoglobin was higher than that of adult hemoglobin both in the presence and absence of 2,3-diphosphoglycerate. The presence of diphosphoglycerate lowers the oxygen affinity of adult hemoglobin much greater than does that of HbF and the diphosphoglycerate levels of red cells of adult and newborn monkeys are about the same. (2) The intensity of the Bohr effect, as expressed by -deltalogP50/deltapH, at pH 7.4 was in the order of fetal hemoglobin-diphosphoglycerate greater than adult hemoglobin-diphosphoglycerate greater than fetal hemoglobin greater than adult hemoglobin.

摘要

从日本猕猴（食蟹猴）中获取成人血红蛋白和胎儿血红蛋白，并研究它们的氧平衡特性。（1）无论有无2,3 - 二磷酸甘油酸，胎儿血红蛋白的氧亲和力均高于成人血红蛋白。二磷酸甘油酸的存在使成人血红蛋白的氧亲和力降低的幅度远大于胎儿血红蛋白，而成猴和新生猴红细胞中的二磷酸甘油酸水平大致相同。（2）在pH 7.4时，以 -deltalogP50/deltapH表示的玻尔效应强度顺序为：胎儿血红蛋白 - 二磷酸甘油酸＞成人血红蛋白 - 二磷酸甘油酸＞胎儿血红蛋白＞成人血红蛋白。