Martínez-Bueno M, Valdivia E, Gálvez A, Maqueda M
Dpto. Microbiología, Facultad Ciencias, Univ. Granada c/Fuentenueva s/n, 18071-Granada, Spain.
Curr Microbiol. 2000 Oct;41(4):257-61. doi: 10.1007/s002840010130.
The complete nucleotide sequence of the small (5149 bp) and cryptic plasmid pS86 from Enterococcus faecalis ssp. faecalis S-86 has been determined. Sequence analysis revealed six putative open reading frames (ORFs) encoding polypeptides of 28.3, 11.5, 8.4, 65.1, 7.3, and 11.96 kDa each. Based on sequence similarity, two cassettes have been identified in pS86: ORF1 codes for the replication initiation protein (Rep); ORF4 codes for a putative mobilization protein that shows similarities to Mob/Pre proteins from plasmids of Gram-positive bacteria. No function could be assigned to the other putative ORFs found. According to our results, pS86 plasmid could use a theta-mode of replication, similar to the recently described theta-type replicons from pUCL287 (Tetragenococcus halophila) and pLA1 or pLA105 (Lactobacillus acidophilus) plasmids.
已确定粪肠球菌亚种粪肠球菌S - 86的小质粒（5149 bp）和隐蔽质粒pS86的完整核苷酸序列。序列分析揭示了六个推定的开放阅读框（ORF），分别编码分子量为28.3、11.5、8.4、65.1、7.3和11.96 kDa的多肽。基于序列相似性，在pS86中鉴定出两个盒式结构：ORF1编码复制起始蛋白（Rep）；ORF4编码一种推定的移动蛋白，该蛋白与革兰氏阳性菌质粒中的Mob/Pre蛋白具有相似性。未发现的其他推定ORF的功能无法确定。根据我们的结果，pS86质粒可能采用θ型复制模式，类似于最近描述的来自pUCL287（嗜盐四联球菌）和pLA1或pLA105（嗜酸乳杆菌）质粒的θ型复制子。
