van der Westhuizen L, Shephard G S, van Schalkwyk D J
Programme on Mycotoxins and Experimental Carcinogenesis, Medical Research Council, Tygerberg, South Africa.
Toxicon. 2001 Feb-Mar;39(2-3):273-81. doi: 10.1016/s0041-0101(00)00125-2.
This is the first report of sphinganine (Sa) and sphingosine (So) levels determined in serum and urine of vervet monkeys (Cercopithecus aethiops) dosed with pure fumonisin B(1) (FB(1)). Initially, experimental vervet monkeys were given a single gavage dose of either 1 or 10 mg FB(1) /kg body weight. Blood and urine were sampled daily and on day seven the monkeys were terminated and the kidneys and livers harvested. In a subsequent experiment, other vervet monkeys were similarly dosed and blood and urine samples were collected over a 50-day period. In the high-dose monkeys the serum Sa/So ratio, as well as levels of serum cholesterol and liver function enzymes, increased during the first week after dosing and remained elevated for several weeks thereafter. The urinary Sa/So ratio and the serum renal function indicators showed a more rapid response and a correspondingly more rapid return to pre-dosing levels. In the low-dose monkeys serum Sa and the Sa/So ratio were the only parameters to increase above the control levels. The Sa/So ratio in liver and kidney tissue showed an elevation over controls in a dose-dependent manner. The serum Sa/So ratio was exclusively elevated above the control levels in the low- and high-dose monkeys and seems more relevant as a marker for fumonisin exposure than any of the other indicators.
这是关于给黑长尾猴（非洲绿猴）投喂纯伏马菌素B1（FB1）后，测定其血清和尿液中鞘氨醇（Sa）和鞘氨醇（So）水平的首份报告。最初，对实验用黑长尾猴单次灌胃给予1或10毫克FB1/千克体重的剂量。每天采集血液和尿液样本，在第7天处死猴子并摘取肾脏和肝脏。在后续实验中，对其他黑长尾猴进行类似给药，并在50天内采集血液和尿液样本。在高剂量组猴子中，给药后第一周血清Sa/So比值以及血清胆固醇和肝功能酶水平升高，此后数周一直维持在较高水平。尿Sa/So比值和血清肾功能指标显示出更快的反应，并相应更快地恢复到给药前水平。在低剂量组猴子中，血清Sa和Sa/So比值是仅有的高于对照水平的参数。肝脏和肾脏组织中的Sa/So比值呈剂量依赖性高于对照组。低剂量和高剂量组猴子的血清Sa/So比值均仅高于对照水平，作为伏马菌素暴露的标志物，它似乎比其他任何指标都更具相关性。
