Moyse E, Lyon M, Cordier G, Mornex J F, Collet L, Froehlich P
Département d'ORL et de Chirurgie Cervico-Faciale, Hôpital E. Herriot, 69003 Lyon, France.
Arch Otolaryngol Head Neck Surg. 2000 Sep;126(9):1105-10. doi: 10.1001/archotol.126.9.1105.
To evaluate viral and cytokine signaling correlates of the persistent inflammation associated with chronic otitis media with effusion (OME).
Prospective study.
Reverse transcriptase-polymerase chain reaction targeting RNA viruses frequently associated with OME (respiratory syncytial virus and parainfluenza virus type 3, the proinflammatory cytokines interleukin 8 and interleukin 1beta, and RANTES [regulated upon activation, normal T cell expressed and secreted]) was performed on mucosal biopsy samples and on samples of the liquid and cellular compartments of inflammatory exudates obtained from 26 children (49 ears) with infected middle ears. Ribonucleic acid extracted from rapidly frozen samples was reverse transcribed by Moloney murine leukemia virus reverse transcriptase and amplified for 35 cycles using previously validated primers. Amplicons were evaluated by molecular size after agarose gel electrophoresis with ethidium bromide.
Most children had evidence of the presence of an RNA virus in at least one specimen. Respiratory syncytial virus was present in 40% and parainfluenza virus type 3 in 8% of effusions. Interleukin 8 messenger RNA was present in 21% of inflammatory exudates but never in cells from the mucosal biopsy samples.
Our data support a viral contribution to the cause of OME and suggest that the inflammatory cytokines observed derive more from cells in the inflammatory exudate than from those in the middle ear mucosa.
评估与慢性分泌性中耳炎（OME）相关的持续性炎症的病毒和细胞因子信号相关性。
前瞻性研究。
对26名患有感染性中耳的儿童（49只耳朵）的黏膜活检样本以及炎性渗出液的液体和细胞成分样本进行逆转录聚合酶链反应，以检测常见于OME的RNA病毒（呼吸道合胞病毒和3型副流感病毒）、促炎细胞因子白细胞介素8和白细胞介素1β以及调节激活正常T细胞表达和分泌因子（RANTES）。从快速冷冻样本中提取的核糖核酸由莫洛尼鼠白血病病毒逆转录酶逆转录，并使用先前验证的引物进行35个循环的扩增。扩增产物在溴化乙锭琼脂糖凝胶电泳后根据分子大小进行评估。
大多数儿童至少有一个样本存在RNA病毒感染的证据。40%的渗出液中存在呼吸道合胞病毒，8%的渗出液中存在3型副流感病毒。21%的炎性渗出液中存在白细胞介素8信使核糖核酸，但在黏膜活检样本的细胞中从未检测到。
我们的数据支持病毒在OME病因中的作用，并表明观察到的炎性细胞因子更多地来源于炎性渗出液中的细胞，而非中耳黏膜中的细胞。
