Woods W G, Whithear K G, Richards D G, Anderson G R, Jorgensen W K, Gasser R B
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria, Australia.
Int J Parasitol. 2000 Aug;30(9):1019-23. doi: 10.1016/s0020-7519(00)00084-9.
Species of Eimeria from chickens from Australia were characterised using a polymerase chain reaction-linked restriction fragment length polymorphism (PCR-RFLP) approach. The ribosomal DNA region spanning the second internal transcribed spacer (ITS-2) was amplified from genomic DNA by PCR, digested separately with three restriction endonucleases (CfoI, Sau3AI and TaqI) and the fragments separated by denaturing gel electrophoresis. The PCR products amplified from the six species varied from approximately 70 to 620 bp on agarose gels, with differences in size and number of bands among species, but no apparent variation within a species. The PCR-RFLP analysis of ITS-2 amplicons on denaturing gels gave characteristic profiles for individual species (except for minor variation in profiles within some species). The results indicate that ITS-2 contains useful genetic markers for the identification of six Eimeria species occurring in Australia.
采用采用�采用聚合酶链反应连锁限制性片段长度多态性（PCR-RFLP）方法对来自澳大利亚鸡的艾美耳球虫种类进行了特征分析。通过PCR从基因组DNA中扩增跨越第二个内部转录间隔区（ITS-2）的核糖体DNA区域，分别用三种限制性内切酶（CfoI、Sau3AI和TaqI）进行消化，并用变性凝胶电泳分离片段。在琼脂糖凝胶上，从这六个种类扩增出的PCR产物大小约为70至620 bp不等，种类之间条带的大小和数量存在差异，但同一种类内无明显变异。对变性凝胶上ITS-2扩增子进行的PCR-RFLP分析给出了各个种类的特征图谱（某些种类内图谱存在微小变异除外）。结果表明，ITS-2包含用于鉴定澳大利亚出现的六种艾美耳球虫种类的有用遗传标记。
