Sklar J, Yot P, Weissman S M
Proc Natl Acad Sci U S A. 1975 May;72(5):1817-21. doi: 10.1073/pnas.72.5.1817.
A major product of the transcription of bacteriophage lambda DNA in vitro is the 6S RNA. This article presents a detailed mapping of restriction endonuclease cleavage sites about the region of the 6S RNA template within the lambda genome. Restriction fragments defined by these sites have been used to localize the 6S RNA template within the physical and genetic maps of the lambda genome. Nucleotide sequence analysis of one of these fragments has largely confimed the nucleotide sequence of the 6S RNA reported previously and has indicated the sequence of DNA that immediately follows the 6S RNA template. This article reports the nucleotide sequence following a known site of transcription termination by RNA polymerase of Escherichia coli.
噬菌体λDNA体外转录的一个主要产物是6S RNA。本文详细绘制了λ基因组中6S RNA模板区域周围的限制性内切酶切割位点图谱。由这些位点定义的限制性片段已被用于在λ基因组的物理图谱和遗传图谱中定位6S RNA模板。对其中一个片段的核苷酸序列分析在很大程度上证实了先前报道的6S RNA的核苷酸序列，并表明了紧跟6S RNA模板之后的DNA序列。本文报道了大肠杆菌RNA聚合酶转录终止已知位点之后的核苷酸序列。
