大肠杆菌O157:H7的脂多糖O-多糖抗原与弗氏柠檬酸杆菌和塞德拉克柠檬酸杆菌菌株之间的血清学交叉反应。

Serological cross-reaction between the lipopolysaccharide O-polysaccharaide antigens of Escherichia coli O157:H7 and strains of Citrobcter freundii and Citrobacter sedlakii.

作者信息

Vinogradov E, Conlan J W, Perry M B

机构信息

Institute for Biological Sciences, National Research Council, Ottawa, Ont., Canada.

出版信息

FEMS Microbiol Lett. 2000 Sep 1;190(1):157-61. doi: 10.1111/j.1574-6968.2000.tb09279.x.

DOI:10.1111/j.1574-6968.2000.tb09279.x

PMID:10981707

Abstract

A strain of Citrobacter sedlakii showing serological cross-reaction with Escherichia coli O157 antisera was demonstrated to produce a lipopolysaccharide O-antigen having an identical structure with that of the E. coli O157 O-antigen. A strain of Citrobacter freunndii showing similar cross-reaction with E. coli O157 specific monoclonal antibody was shown to produce a lipopolysaccharide O-antigen composed of a trisaccharide repeating unit having the structure [ 2)-alpha-D Rhap-(1-3)-beta-D-Rhap-(1-4)-beta-D-Glcp-(1-]. This O-antigen differs from that of the E. coli O157 O-antigen and also lacks a component 2-substituted 4-amino-4,6-dideoxy-alpha-D-mannopyranosyl residue implicated as the common epitope in the lipopolysaccharide O-antigens of previously investigated bacterial species showing serological cross-reactivity with E. coli O157 antisera. The C freundii O-antigen presents an interesting example of structural mimicry within a bacterial polysaccharide antigen.

摘要

一株与大肠杆菌O157抗血清呈现血清学交叉反应的塞德莱克柠檬酸杆菌被证明产生一种脂多糖O抗原，其结构与大肠杆菌O157的O抗原相同。一株与大肠杆菌O157特异性单克隆抗体呈现相似交叉反应的弗氏柠檬酸杆菌被证明产生一种脂多糖O抗原，该抗原由具有[2)-α-D-鼠李糖-(1-3)-β-D-鼠李糖-(1-4)-β-D-葡萄糖-(1-]结构的三糖重复单元组成。这种O抗原不同于大肠杆菌O157的O抗原，并且还缺少一种成分，即2-取代的4-氨基-4,6-二脱氧-α-D-甘露吡喃糖基残基，该残基被认为是先前研究的与大肠杆菌O157抗血清呈现血清学交叉反应的细菌物种的脂多糖O抗原中的共同表位。弗氏柠檬酸杆菌O抗原是细菌多糖抗原内结构模拟的一个有趣例子。