DNA拓扑异构酶I、IIα和IIβ在人脑肿瘤中的表达。

Expression of DNA topoisomerase I, IIalpha, and IIbeta in human brain tumors.

作者信息

Morisaki K, Kuroda S, Matsumoto Y, Kunishio K, Nagao S

机构信息

Department of Neurological Surgery, Kagawa Medical University, Kitagun, Japan.

出版信息

Brain Tumor Pathol. 2000;17(1):7-13. doi: 10.1007/BF02478912.

DOI:10.1007/BF02478912

PMID:10982004

Abstract

We investigated the expression of DNA topoisomerase I (Topo I), IIalpha (Topo IIalpha), and IIbeta (Topo IIbeta) mRNA using reverse transcription-polymerase chain reaction (RT-PCR) assay in 31 human brain tumors, and examined the relationship between DNA topoisomerase mRNA expression and Topo IIalpha and MIB-1 positive index (PI) as a cell proliferation marker. Topo IIalpha mRNA was expressed in 11 of 31 cases, and Topo I and IIbeta were each expressed in 18 of 31 cases. A significant correlation was seen between the MIB-1 PI and Topo IIalpha PI (P < 0.001). The cases with overexpression of Topo IIalpha mRNA had significantly high MIB-1 and Topo IIalpha PI (P < 0.0001). There was no significant correlation between Topo I and IIbeta mRNA expression and MIB-1 PI. We concluded that it was useful as a cell proliferation marker to analyze the expression of Topo IIalpha mRNA using RT-PCR in human brain tumors.

摘要

我们采用逆转录聚合酶链反应（RT-PCR）检测法，对31例人脑肿瘤中DNA拓扑异构酶I（Topo I）、IIα（Topo IIα）和IIβ（Topo IIβ）mRNA的表达进行了研究，并探讨了DNA拓扑异构酶mRNA表达与作为细胞增殖标志物的Topo IIα和MIB-1阳性指数（PI）之间的关系。31例病例中，11例表达Topo IIα mRNA，31例中各有18例表达Topo I和IIβ mRNA。MIB-1 PI与Topo IIα PI之间存在显著相关性（P < 0.001）。Topo IIα mRNA过表达的病例具有显著较高的MIB-1和Topo IIα PI（P < 0.0001）。Topo I和IIβ mRNA表达与MIB-1 PI之间无显著相关性。我们得出结论，在人脑肿瘤中，利用RT-PCR分析Topo IIα mRNA的表达作为细胞增殖标志物是有用的。