Peters R R, Komaragiri S, Paape M J, Douglass L W
Department of Animal and Avian Sciences, University of Maryland, College Park, 20742-2311, USA.
J Dairy Sci. 2000 Aug;83(8):1750-7. doi: 10.3168/jds.S0022-0302(00)75045-4.
We evaluated the effectiveness of a 1.6% phenol-based teat dip using both a teat skin assay and natural exposure field trial. A teat skin assay was conducted to ascertain the concentration of phenol + phenate to be used in the field study. One percent and 0.5% iodine, and 0.5, 1.1, and 1.6% phenol + phenate were compared using Escherichia coli and Staphylococcus aureus. Logarithmic reductions for S. aureus were 2.2 and 2.8 for 0.5 and 1% iodine, and 1.3, 2.1, and 2.8 for 0.5, 1.1, and 1.6% phenol + phenate, respectively. Logarithmic reductions for E. coli were 3.3 and 3.8 for 0.5 and 1% iodine, and 1.2, 1.9, and 2.6 for 0.5, 1.1, and 1.6% phenol + phenate, respectively. A concentration of 1.6% phenol + phenate was chosen as experimental teat dip, and 0.5% iodine served as control. The field study was conducted at Beltsville (n = 185) and Clarksville (n = 100) dairy herds using a split herd design. Teat dips were used premilking and postmilking for 12 mo. The number of new intramammary infections (IMI) for the Beltsville herd in iodine and phenol + phenate teat dipped cows were: 29 and 35 for major pathogens, and 81 and 72, for minor pathogens. For the Clarksville herd, number of new intramammary infections in iodine and phenol + phenate teat dipped cows was 9 and 10 for major pathogens, and 50 and 60 for minor pathogens. Rates of IMI per quarter day per lactation were not different for either herd or when herd data were combined. The number of clinical mastitis cases per 100 cows per month were similar in both treatments. The incidences of new IMI and clinical mastitis were similar using both dips.
我们通过乳头皮肤试验和自然暴露田间试验评估了一种1.6%的酚基乳头浸液的有效性。进行乳头皮肤试验以确定田间研究中使用的酚+酚盐的浓度。使用大肠杆菌和金黄色葡萄球菌比较了1%和0.5%的碘，以及0.5%、1.1%和1.6%的酚+酚盐。金黄色葡萄球菌的对数减少率在0.5%和1%碘时分别为2.2和2.8，在0.5%、1.1%和1.6%酚+酚盐时分别为1.3、2.1和2.8。大肠杆菌的对数减少率在0.5%和1%碘时分别为3.3和3.8，在0.5%、1.1%和1.6%酚+酚盐时分别为1.2、1.9和2.6。选择1.6%的酚+酚盐浓度作为试验性乳头浸液，0.5%的碘作为对照。田间研究在贝尔茨维尔（n = 185）和克拉克斯维尔（n = 100）奶牛场采用分群设计进行。乳头浸液在挤奶前和挤奶后使用12个月。在贝尔茨维尔牛群中，用碘和酚+酚盐浸液处理的奶牛新发生的乳房内感染（IMI）数量，主要病原体分别为29例和35例，次要病原体分别为81例和72例。在克拉克斯维尔牛群中，用碘和酚+酚盐浸液处理的奶牛新发生的乳房内感染数量，主要病原体分别为9例和10例，次要病原体分别为50例和60例。无论是单个牛群还是合并牛群数据，每泌乳期每季度日的IMI发生率均无差异。两种处理方式下每月每100头奶牛的临床乳腺炎病例数相似。两种浸液的新IMI和临床乳腺炎发生率相似。
