兵豆（Lens culinaris Medik.）与其他豆科植物物种rDNA基因间隔区结构的比较研究。

A comparative study of the structure of the rDNA intergenic spacer of Lens culinaris Medik., and other legume species.

作者信息

Fernández M, Polanco C, Ruiz M L, Pérez de la Vega M

机构信息

Departamento de Genética, Facultad de Biología, Universidad de León, Spain.

出版信息

Genome. 2000 Aug;43(4):597-603. doi: 10.1139/g00-022.

DOI:10.1139/g00-022

PMID:10984170

Abstract

As part of a project on lentil molecular genetics, the sequence of the 18S-25S ribosomal RNA gene intergenic spacer (IGS) of Lens culinaris Medik. was determined. DNA was cloned after polymerase chain reaction (PCR) amplification. The spacer of 2939 bp was composed of nonrepetitive sequences and four tandem arrays of repeated sequences, named A to D. C and D arrays were formed by the repetition of very short consensus sequences. Similarity was found between lentil and other legume species, in particular those of the Vicieae tribe. A transcription initiation site, putative sites of termination and processing, and promoter-enhancer sequences were detected by computer-aided searches. These sites resemble motifs conserved in the IGS sequences of other plant species. The conservation of motifs in the otherwise highly variable plant IGS sequences points to the relevance of these motifs as functional sequences.

摘要

作为小扁豆分子遗传学项目的一部分，测定了栽培小扁豆（Lens culinaris Medik.）18S - 25S核糖体RNA基因间隔区（IGS）的序列。经聚合酶链反应（PCR）扩增后对DNA进行了克隆。2939 bp的间隔区由非重复序列和四个重复序列串联阵列组成，命名为A至D。C和D阵列由非常短的共有序列重复形成。在小扁豆与其他豆科物种，特别是野豌豆族的物种之间发现了相似性。通过计算机辅助搜索检测到一个转录起始位点、假定的终止和加工位点以及启动子 - 增强子序列。这些位点类似于其他植物物种IGS序列中保守的基序。在其他方面高度可变的植物IGS序列中基序的保守性表明这些基序作为功能序列的相关性。