Galasso Incoronata
Istituto di Genetica Vegetale, CNR, Bari, Italy.
Genome. 2003 Dec;46(6):1118-24. doi: 10.1139/g03-077.
Multiple-target fluorescence in situ hybridization (FISH) was applied on mitotic chromosomes of seven Lens taxa using two highly repetitive sequences (pLc30 and pLc7) isolated from the cultivated lentil and the multigene families for the 18S-5.8S-25S (pTa71) and 5S rRNA (pTa794) from wheat simultaneously as probes. The number and location of pLc30 and pLc7 sites on chromosomes varied markedly among the species, whereas the hybridization pattern of 5S rDNA and 18S-5.8S-25S rDNA was less variable. In general, each species showed a typical FISH karyotype and few differences were observed among accessions belonging to the same species, except for the accessions of Lens odemensis. The most similar FISH karyotype to the cultivated lentil is that of Lens culinaris subsp. orientalis, whereas Lens nigricans and Lens tomentosus are the two species that showed the most divergent FISH patterns compared with all taxa for number and location of pLc30 and 18S-5.8S-25S rDNA sites.
利用从栽培小扁豆中分离出的两个高度重复序列（pLc30和pLc7）以及来自小麦的18S - 5.8S - 25S（pTa71）和5S rRNA（pTa794）多基因家族作为探针，对七个小扁豆分类群的有丝分裂染色体进行了多靶点荧光原位杂交（FISH）。pLc30和pLc7位点在染色体上的数量和位置在不同物种间差异显著，而5S rDNA和18S - 5.8S - 25S rDNA的杂交模式变化较小。总体而言，每个物种都呈现出典型的FISH核型，除了奥德曼斯小扁豆的种质外，同一物种的种质间观察到的差异很少。与栽培小扁豆最相似的FISH核型是东方小扁豆的，而黑小扁豆和绒毛小扁豆是与所有分类群相比，在pLc30和18S - 5.8S - 25S rDNA位点的数量和位置上显示出最不同FISH模式的两个物种。
